The number and nature of glucose-phosphorylating enzymes of rat intestinal mucosa were investigated by chromatographic, electrophoretic, and kinetic methods. Three fractions with glucose-phosphorylating activity were obtained from the supernatant fluid of mucosa homogenate by means of DEAE-cellulose chromatography, corresponding to hexokinases A and B (EC 2.7.1.1.), and N-acetyl-D-glucosamine k...

J1P NMR studies with Cd(II) and Zn(II) chelates of adenosine 5’-0-(34hiotriphosphate) (ATPyS) and the Cd(D) chelate of adenosine 5’-O-(24hiotriphosphate) (ATPBS) indicate that these metal ions chelate to the sulfur atom of the thiophosphate group. Since Mg(II) chelates to oxygen of the thiophosphate group of ATPjIS, the configuration of the Mg(II) chelate of one diastereoisomer is equivalent to...

BACKGROUND Secretion of histotroph during the prolonged pre-implantation phase in mares is crucial to pregnancy maintenance, manifested as increased embryonic loss in mares with age-related endometrial degeneration. Glycogen content of uterine histotroph is higher during the progesterone-dominated phase of the estrous cycle in mares, but regulatory mechanisms are not well understood. METHODS ...

The enzyme which catalyzes the phosphorylation of glucose and fructose was described in 1927 by Meyerhof (1). He found that muscle extracts which split polysaccharides and hexose phosphates to lactic acid but were unable to ferment hexoses could be enabled to ferment glucose and fructose by addition of a protein fraction from bakers’ yeast. The same protein fraction activated the aerobic oxidat...

Thesis Abstract Hexokinase 2 enzyme catalyzes the rate-limiting step in glycolysis. It is overexpressed several carcinomas including breast cancer to sustain energy for rapidly dividing cells and associates with chemoresistance. However, impact of chemo drugs alone or combination on hexokinase activity autophagic cell death unclear. Arsenic trioxide (As2O3) (ATO) a metalloid potent antineoplast...