I have been using the spectrophoto-metric assay of Cushman and Cheung (1) as modified by Lieberman (2) to assay for serum angiotensin-converting enzyme (ACE) in diagnosis of possible sarcoidosis. However, replicate determinations produced results that varied considerably, creating some doubt about its analytical usefulness. Taylor and Freeman (3) identified a cause of this variability as cloudi...

In this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously developed approach, pentacyanoferrate was coordinated with pyridyl-N ligands, and the redox-active...

Print workers are exposed to organic solvents, of which the systemic toxicant toluene is a main component. Toluene induces expression of cytochrome P450 2E1 (CYP2E1), an enzyme involved in its own metabolism and that of other protoxicants, including some procarcinogens. Therefore, we investigated the association between toluene exposure and the CYP2E1 response, as assessed by mRNA content in pe...

It has been postulated that occupational exposure to hydrocarbons may damage the kidney and lead to glomerulonephritis and chronic renal failure. As laminin is a ubiquitous basement membrane component that seems to play a central part in the structure and function of basement membranes and as the normal renal filtration process is highly dependent on an intact glomerular basement membrane, the ...

BACKGROUND The aims of this study were evaluation of exposed to xylenes in low concentration and compare urinary level of methyl hippuric acid in taxi drivers and petrol stations workers in West of Iran. METHODS This observation study was carried out on samples of the exposed men to xylenes in two occupational groups in Hamadan City (west of Iran) from March 2003 to March 2004. Subjects inclu...

At present, the clinical significance of existing physicochemical and biological evidence and especially the results we have obtained from our previous in vitro experiments have been analyzed, and we have come to the conclusion that hippuric acid (C6H5CONHCH2COOH) is a very active solvent of Calcium Oxalate (CaOX) in physiological solutions. Two types of experiments have been discussed: clinica...

An enzyme which catalyzes the aromatization of cyclohexanecarboxyl coenzyme A has been isolated from whole liver as well as from liver mitochondria. Incubation of the soluble, partially purified enzyme with cyclohexanecarboxyl-CoA produces cyclohexene-l-carboxyl-CoA, benzoyl-CoA, and a polar compound which has not. been further characterized. All three isomers of cyclohexenecarboxyl-CoA are con...

In spite of a large number of investigations, one encounters in the current literature the most diverse statements concerning the fate of salts of benzoic acid in the human organism. Only one fact is apparently accepted by all as settled beyond question, namely, that part of the benzoate is converted into a hippurate through union with glycocoll. Upon the question as to horn nearly this reactio...