Immunotherapy, particularly the adoptive cell transfer (ACT) of tumor-infiltrating lymphocytes (TIL), is a very promising therapy for metastatic melanoma. Some patients unable to receive TIL have been successfully treated with autologous peripheral blood lymphocytes (PBL), genetically modified to express human leukocyte antigen (HLA) class I antigen-restricted, melanoma antigen-reactive T-cell ...

the high molecular weight glutenin subunit (hmw-gs) compositions of 19 diploid wild wheat (t.boeoticum) and 36 diploid wild wheat (a.tauschii) were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). all of t. boeoticum were characterized for their hmw-gs, showed 2* in their glu-a1 locus. in the study of aegilops tauschii that were collected from iran and 7 neig...

Background Excessive accumulation of amyloid β-protein (Aβ) is one the primary mechanisms that leads to neuronal death with phosphorylated tau in pathogenesis Alzheimer’s disease (AD). Protofibrils, high-molecular-weight Aβ oligomers (HMW-Aβo), are implicated be important targets modifying therapy AD. We previously reported phenolic compounds such as myricetin inhibit Aβ1-40, Aβ1-42, and α-synu...

The presence of white matter lesions (WML) is an important prognostic factor for the development of stroke in patients with type 2 diabetes (1). Adiponectin, especially high molecular weight (HMW) adiponectin, an adipocyte-derived peptide with antidiabetic and antiatherogenic effects, is known to protect against the initiation and progression of diabetes, atherosclerosis, and insulin resistance...

The realization that tumor cells utilize multiple mechanisms to escape from immune recognition and destruction has stimulated interest in developing and applying immunotherapeutic strategies which target both humoral and cellular immunity to malignant cells. As a result, the tumor-associated antigens (TAA) used as targets have to be expressed on the cell surface membrane of malignant cells. Fur...

Several tumor cells secrete significantly increased amounts of the plasminogen activator urokinase, a trypsinlike serine protease, whose biological function in tumor biology is unclear. In this study we report that cells of the human epidermal tumor cell line CCL 20.2 express about 80,000 high-affinity urokinase receptors per cell that bind active as well as diisopropylfluorophosphate-treated h...

Five commercial wheat varieties, namely C 306, HI 977, HW 2004, PBW 550 and WH 542 of diverse baking performances were examined for their physicochemical parameters, gluten quality and HMW-GS composition. All the varieties presented a broad spectrum of different quality parameters.Based on the kernel dimensions, thousand kernel weight and hectolitre weight, WH 542 was observed to have smaller a...

Background and Objectives Wheat gluten proteins make up one of the most complex protein aggregates in nature. Their qualitative quantitative composition is determined by genetic environmental factors as well technological processes. Findings Gluten comprise ?5-, ?1,2-, ?-, ?-gliadins high-molecular-weight glutenin subunits (HMW-GS) low-molecular-weight (LMW) GS. About 50% are monomeric gliadins...

The effects of the rolling-circle and theta modes of replication on the maintenance of recombinant plasmids in Lactococcus lactis were studied. Heterologous Escherichia coli or bacteriophage lambda DNA fragments of various sizes were inserted into vectors based on either the rolling-circle-type plasmid pWV01 or the theta-type plasmid pAMbeta1. All pAMbeta1 derivatives were stably maintained. pW...