Background: Infection with Escherichia coli O157:H7 rarely leads to bloody diarrhea and causes hemolytic uremic syndrome with renal failure that can be deadly dangerous. Intimin, translocated Intimin receptor (Tir), and enterohemorrhagic E. coli (EHEC) secreted protein A (EspA) proteins are the virulence factors expressed by locus of enterocyte effacement locus of EHEC. This bacterium needs Es...

The functional elucidation of small unknown phage proteins ('ORFans') presents itself as one of the major challenges of bacteriophage molecular biology. In this work, we mined the Pseudomonas aeruginosa-infecting phage LUZ24 proteome for antibacterial and antibiofilm proteins against its host. Subsequently, their putative host target was identified. In one example, we observed an interaction be...

Herpes simplex virus (HSV) stimulates DNA synthesis in mouse spleen cultures prepared from normal, macrophage-depleted, and T-cell-depleted spleen cells, but not from thymocytes. In addition, a polyclonal antibody response is observed in HSV-infected spleen cultures. These findings indicate that the cells stimulated to undergo DNA synthesis after HSV infection appear to be the bone marrow-deriv...

conclusions the results revealed that the novel multi-epitope dna construct could be an effective candidate in tuberculosis vaccine development, and it is qualified to investigate its potential to induce cd4 and cd8 t- cell immune response in the experimental animal model. results mhc class i-and class ii-predicted epitopes showed a high potential to binding to human hlas alleles, with global b...

background: benzene is one of the important occupational pollutants. there are some reports about the leukemogenic effects related to low-level exposure to benzene. objective: to study the frequency of micronucleus (mn), nucleoplasmic bridge (nb), and nuclear budding (nbud) in the peripheral blood lymphocytes of petrochemical workers with low level exposure to benzene. methods: we enrolled 50 w...

Gene sequences in human oocytes were studied to investigate the possibility of diagnosing inherited or sporadic genetic disease before implantation after in vitro fertilisation. By specific amplification the possibility of analysing the DNA from single human oocytes for a specific gene was shown, and genotypes for markers closely linked to cystic fibrosis and Duchenne muscular dystrophy were de...

T he main hurdle for genome sequencing projects these days is no longer the effort and cost of generating sequence data—which has become exponentially cheaper—but the capacity to analyse huge amounts of data and make sense of it. This endeavour is made harder by another problem that has begun to emerge over the past years: DNA contamination. Contamination impacts both sequence data generation, ...