Incorporation of unnatural amino acids into recombinant proteins represents a powerful tool for protein engineering and protein therapeutic development. While the processing of the N-terminal methionine (Met) residues in proteins is well studied, the processing of unnatural amino acids used for replacing the N-terminal Met remains largely unknown. Here we report the effects of the penultimate r...

This work aims to verify whether Galvanic Skin Response (GSR) can be used as a valid pain indicator and to analyze the changes in GSR during hypnotic analgesic condition. Two types of experiments were conducted namely ‘Pain-GSR’ (PG) and ‘Hypnosis-Pain-GSR’ (HPG) experiments for 10 subjects. The experiments were done under pain and no-pain conditions on subjects of both the gender in the age gr...

El objetivo de esta investigación fue determinar la prevalencia parásitos gastrointestinales en caballos durante el periodo cuarentena 2011 Hipódromo “La Rinconada”, Caracas, Venezuela. Se realizó un estudio coprológico por técnica flotación Mc master (Willis-Molloy) a total 894 equinos (Equus caballus), raza Pura Sangre Carrera, 400 sexo macho y 494 hembras, todos 2 años edad, procedentes cent...

Temperature-triggered copolymers are proposed for a number of bio-applications but there is no ideal material platform, especially for injectable drug delivery. Options are needed for degradable biomaterials that not only respond to temperature but also easily accommodate linkage of active molecules. A first step toward realizing this goal is the design and synthesis of the novel materials repo...

Puberty is a complex process that culminates in the acquisition of psychophysical maturity and reproductive capacity. This elaborate fascinating marks end childhood. Behind it lies complex, genetically mediated neuroendocrine mechanism through which gonads are activated thanks to fine balance between central inhibitory stimulating neuromodulators hormones with both peripheral action. The onset ...

nano sio2/hyperbranched polyglycerol (hpg)/so3h catalyst was readily prepared for first time from inexpensive starting materials in aqueous media which catalyzed the synthesis of benzoic acid. ftir spectra, x-ray diffraction (xrd) and transmission electron micrographs (tem) was employed to characterize the properties of the synthesized nano sio2/hpg/so3h. high catalytic activity and ease of rec...

Over the last few years, numerous studies solidified the hypothesis that fibroblast growth factor (FGF) signaling regulates neuroendocrine progenitor cell proliferation, fate specification, and cell survival and, therefore, is critical for the regulation and maintenance of homeostasis of the body. One important example that underscores the involvement of FGF signaling during neuroendocrine cell...

PURPOSE The present work describes the development and in vitro and in vivo evaluation of a mucoadhesive nanoparticulate docetaxel (DTX) formulation for intravesical bladder cancer therapy. EXPERIMENTAL DESIGN Mucoadhesive formulations based on hyperbranched polyglycerols (HPG), hydrophobically derivatized with C(8)/C(10) alkyl chains in the core and modified with methoxy-polyethylene glycol ...

To establish peptide vaccine-based cancer immunotherapy, we investigated the improvement of antigenic peptides by encapsulation with pH-sensitive fusogenic polymer-modified liposomes for induction of antigen-specific immunity. The liposomes were prepared by modification of egg yolk phosphatidylcholine and l-dioleoyl phosphatidylethanolamine with 3-methyl-glutarylated hyperbranched poly(glycidol...

Abnormal sperm production and reduced fertility have been reported in transgenic male mice lacking the alpha-subtype of the estrogen receptor (ER)alpha or aromatase. The aim of this study was to investigate the role of estrogen in male reproductive function, by determining the effect of estradiol on testicular function in hypogonadal (hpg) mice congenitally lacking gonadotropin; and thus, sex s...