Previously, we reported that tasar silkworm pupal oil (TPO) is a rich source of alpha-linolenic acid (ALA) (37%). In this study, have used three chromatographic techniques, namely preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), and chromatography flame ionization detection (GC-FID), to purify, determine, quantify (ALA), respectively, from TPO. Additionally, determine the potenti...

A HPLC method with UV detection for quantification of 2,6-diaminopimelic acid (DAPA) in rumen bacteria, duodenal and ileal digesta and faeces is described. Biological samples were hydrolyzed with 6 M HC1 for 20 h at 104±2°C. DAPA was separated after pre-column derivatization with o-phthaldialdehyde (OPA) in the presence of ethanethiol (ESH). DAPA derivative was analyzed using a reversed-phase C...

Abstract Here, two important isoflavones present in Trifolium pratense, formononetin and biochanin A, were analyzed by high performance liquid chromatography coupled with UV (HPLC–UV). These are marker compounds anti-inflammatory, anti-cancer, anti-oxidant properties, also potent phytoestrogens that can be used hormonal therapies. Till date, no study regarding rapid identification quantificatio...

In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatographic separat...

A high-performance liquid chromatographic (HPLC) method with UV and DAD detection for the quantitative determination of linezolid in human serum was developed in present work. Chromatography was carried out by reversed-phase technique on a RP-18 column with a mobile phase composed of 50 mM phosphate buffer and acetonitrile (76 : 26, v/v), adjusted to pH 3.5 with orthophosphoric acid. Serum samp...

BACKGROUND The potent immunosuppressant mycophenolic acid (MPA) is metabolized to an inactive glucuronide (MPAG). The extent of metabolism varies among individuals, and the MPAG formed can be hydrolyzed to MPA and can displace MPA from serum albumin, creating a potential need to monitor both MPA and MPAG. METHODS After addition of the carboxybutoxy ether of MPA (MPAC) as internal standard, MP...

To evaluate cellular uptake and purine transport, we developed a high-performance liquid chromatography method for intra- and extracellular purine quantification. Our aim was to develop an effective method for simultaneously quantifying the substrate and metabolites with high sensitivity. C18 columns from different manufacturers were tested for simultaneous quantification of 22 different purine...

Mass spectrometry (MS) is an analytical technique which is used to measure sample’s molecular mass by measuring mass to charge ratio. In this method, a soft ionization technique like ESI (Electro Spray Ionization) is used to generate charge ions. Now days, this is the most popular method for protein identification and quantitation. In shotgun proteomics proteins are digested into smaller peptid...