the mrna expression of growth hormone (gh) gene in early development stages of siberian sturgeon was investigated using rt-pcr method. samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. ribosomal protein l6 (rpl6) transcripts were used as the internal standard during quantification of gh mrna expression. the results showed that the gh mr...

recombinant human growth hormone (hgh) was given for 6 months or longer to 68 patients with gh deficiency, 6 with turner's syndrome and 5 with achondroplasia, during 1986-1994 in a prospective study. the total weekly administered dose of gh was 0.6 u/kg. by random sampling, 29 of 68 gh deficient patients (42.6%) received twice weekly (2 iw) intramuscular (im) injections, 17 (25%) received ...

Human recombinant erythropoietin is a synthetic hormone for treatment of some kinds of anemia. In this study, the effect of this hormone was investigated on blood cell changes in adult and immature rats. For this purpose, the hormone was administered at doses of 50, 100, and 200 IU/Kg (s.c.) twice a week for a period of 6 weeks. Then, the number of red blood cells (RBC), subtypes of white blood...

The purpose of this study was to investigate the involvement of growth hormone in the diurnal variation of insulin sensitivity in healthy adults. Afternoon (16:00 hr) or night (23:00 hr) pretreatment with a subcutaneous injection of normal saline, human growth hormone to mimic the normal nocturnal rise in growth hormone, or octreotide to inhibit endogenous growth hormone secretion to create a s...

Growth is impaired in a chronic renal failure. Anemia, acidosis, reduced intake of calories and protein, decreased synthesis of vitamin D and increased parathyroid hormone levels, hyperphosphatemia, renal osteodystrophy and changes in growth hormone-insulin-like growth factor and the gonadotropin-gonadal axis are implicated in this study. Growth is adversely affected by immunosuppressives and c...

A number of attempts have been made to measure the amount of growth hormone in human plasma or in extracts thereof. By using the tibia-line biological assay Gemzell (1959) and Forsman & Gemzell (1959) detected growth hormone in extracts from subjects with acromegaly or diabetes but not in extracts of normal plasma. Owing to the lack of sensitivity and specificity of biological assays for growth...

A binding study of nickel ions by a new recombinant human Growth Hormone (hGH), produced as an injected drug, has been done at 27˚C in NaCl solution (50 mM) using an isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for nickel ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μM and -16...

The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for  and , and also their molar enthalpies of binding (KJ/mol for  and  KJ/mo...