BACKGROUND Chemo-resistance is a leading cause of tumor relapse and treatment failure in patients with ovarian cancer. The identification of effective strategies to overcome drug resistance will have a significant clinical impact on the disease. METHODS The protein and mRNA expression of GOLPH3L in ovarian cancer cell lines and patient tissues were determined using Real-time PCR and Western b...

BACKGROUND Ovarian tissue cryopreservation may be a potential method of preserving fertility in women who have experienced gonadotoxic treatments. To improve the efficiency of existing cryopreservation, we developed a practical and convenient vitrification method named needle immersed vitrification (NIV), which required a less concentrated and minimum volume of vitrification solution. METHODS...

Background/aim: Ovarian transplantation can preserve fertility in cancer patients. The aim of this study was to compare ovarian transplantation into granulation tissue (GT) versus back muscle (BM) sites and also to investigate the effects of N-acetylcysteine (NAC) as an antioxidant on ovarian survival in a rat autograft model.Materials and methods: Twenty-eight adult female rats were divided in...

Inflammation has recently been implicated in cancer formation and progression. As tissue transglutaminase (TG2) has been associated with both inflammatory signaling and tumor cell behavior, we propose that TG2 may be an important link inducing interleukin-6 (IL-6)-mediated cancer cell aggressiveness, including cancer stem cell-like characteristics and distant hematogenous metastasis. We evaluat...

INTRODUCTION The polycomb group (PcG) proteins form chromatin-modifying complexes that are commonly deregulated in cancer. The PcG protein BMI-I is overexpressed by various tumours and thus may contribute to malignant transformation. The current study investigated the expression of BMI-I in human specimens of breast, ovarian, endometrial and cervical cancer. MATERIALS AND METHODS Expression o...

BACKGROUND Ovarian tissue cryopreservation, in combination with autotransplantation or long-term culture, has been proposed as a means of fertility preservation. However follicle density within ovarian cortex has a profound impact on the success of in vivo and in vitro systems designed to support follicle growth and restore fertility. The objective of this study was to investigate the dye neutr...

The goals and rhetoric of The Oncofertility Consortium [1] are aimed toward preserving cancer patients’ reproductive choices and facilitating their reproductive autonomy after cancer. While the end goals of oncofertility research are oriented toward safeguarding the possibility of biological reproduction for women and girls facing cancer treatments that may affect their reproductive capacity, c...

Eight different human ovarian carcinomas were shown to have 2 to 25 times higher transfer RNA methylase-specific activity and transfer RNA methylating capacity than do normal ovarian tissues. The more rapidly metastasizing, poorly differentiated carcinomas had higher transfer RNA methylase activity than did slower metastasizing, well-dif ferentiated, and intermediately differentiated carcinomas...

Endometriosis is a condition that is characterized by the presence of endometrial tissue implants that are outside the uterine lining. It is presented in 10–15% of women in the reproductive age group, 2–5% of postmenopausal women, 25–30% of infertile women and 40–70% of women with chronic pelvic pain. The natural course of endometriosis starts with ectopic tissue implantation and bleeding occur...

BACKGROUND Twist2 has been shown to promote human tumor invasion as in breast cancer and cervical cancer. However, whether Twist2 promotes human ovarian cancer progression remains to be elucidated. Here, we investigate the role of Twist2 in ovarian cancer invasion and metastasis as well as the underlying molecular mechanisms. METHODS Twist2 expression was detected by Immunohistochemistry (IHC...