Human-induced pluripotent stem (iPS) cells share the same key properties as embryonic stem cells, and may be generated from patient- or disease-specific sources, which makes them attractive for personalized medicine, drug screens, or cellular therapy. Long-term cultivation and maintenance of normal iPS cells in an undifferentiated self-renewing state is a major challenge. Our previous studies h...

research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

The spermatogonial stem cells (SSCs) are at the foundation of spermatogenesis. They can self-renew and generate a large number of differentiated germ cells. A balance between SSC renewal and differentiation in the adult testis is essential to maintain normal spermatogenesis and fertility. These two processes are tightly regulated by intrinsic gene expression in the stem cells and extrinsic sign...

identification of exogenous factors affecting spermatogonial stem cells (sscs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. the aim of this study was to investigate the effects of a gnrh analogue (alareline acetate) on sscs colonization in short-term co-culture with sertoli cells. five, three-month old holstein male calves were used to isolate spermatogo...