Hyaluronan is made and extruded from cells to form a pericellular or extracellular matrix (ECM) and is present in virtually all tissues in the body. The size and form of hyaluronan present in tissues are indicative of a healthy or inflamed tissue, and the interactions of hyaluronan with immune cells can influence their response. Thus, in order to understand how inflammation is regulated, it is ...

We recently purified the rat liver hyaluronan receptor for endocytosis (HARE) and found abundant expression of 175- and approximately 300-kDa HARE species in sinusoidal endothelial cells of the liver, spleen, and lymph nodes. We report herein the first cloning and functional expression of the rat 175-kDa HARE. Peptide sequences were obtained from the purified 175-kDa HARE, and degenerate oligon...

Chondrocytes were isolated from adult laryngeal cartilage by an enzymic procedure that included 6 h digestion with collagenase. The level of 35SO4(2-) incorporation into cetylpyridinium chloride-precipitable material by these cells depended upon the subsequent culturing conditions. Suspension cultures incorporated more 35SO4(2-)/cell than monolayer cultures. Hyaluronic acid in the medium inhibi...

BACKGROUND Following tissue injury, monocytes can enter the tissue and differentiate into fibroblast-like cells called fibrocytes, but little is known about what regulates this differentiation. Extracellular matrix contains high molecular weight hyaluronic acid (HMWHA; ∼2×10(6) Da). During injury, HMWHA breaks down to low molecular weight hyaluronic acid (LMWHA; ∼0.8-8×10(5) Da). METHODS AND ...

CONTEXT Intra-articular hyaluronic acid is a US Food and Drug Administration-approved treatment for knee osteoarthritis (OA); however, its efficacy is controversial. OBJECTIVE To evaluate whether intra-articular hyaluronic acid is efficacious in treating knee OA. DATA SOURCES We searched for human clinical trials in MEDLINE (1966 through February 2003) and the Cochrane Controlled Trials Reg...

PURPOSE To study the effect of acute or chronic intracameral injection of hyaluronic acid on intraocular pressure (IOP) in rats. METHODS Acute or chronic injections of hyaluronic acid were performed unilaterally in the rat eye's anterior chamber, whereas the contralateral eye was injected with saline solution. IOP was assessed daily or weekly by a tonometer in conscious rats. IOP was also ass...

AN attempt has been made to find the cells of origin and the time of origin during development of the vitreous hyaluronic acid. The adult vitreous body of those species so far examined seems to owe its characteristic jelly-like properties to the presence of small amounts of an insoluble collagen-like protein laid down in fibrous lamellae and a low concentration of hyaluronic acid. Earlier work ...

Injectable filling agents offer the promise of a better appearance without surgery and, among them, hyaluronic acid is the most commonly used. Although complications are rare, it is necessary to know the possible side effects and complications in order to be prepared for their management. That is why many researchers have been focusing on the interactions between hyaluronic acid and pathogens, ...

BACKGROUND Bioengineered hyaluronic acid derivatives are currently available that provide for safe and effective soft-tissue augmentation in the comprehensive approach to nonsurgical facial rejuvenation. Current hyaluronic acid fillers do not require preinjection skin testing and produce reproducible, longer-lasting, nonpermanent results compared with other fillers, such as collagen. METHODS ...