THE effect of hyaluronidase injections on the slit-lamp appearance and the composition of the vitreous humour of the rabbit has been examinfed. The purpose of the experiments has been to see whether disaggregation of hyaluronic acid from a viscous to a non-viscous form in the vitreous humour is a permanent change and whether any visible change persists as a result of hyaluronidase action. Very ...

The effect of glycosaminoglycan addition on a three-dimensional (3D) culture of porcine chondrocyte cells was investigated with a view to use in cartilage regenerative medicine. Chondroitin sulfate C increased the mRNA expression of type 2 collagen, while chondroitin sulfate A did not. Hyaluronic acid of high molecular weight markedly decreased the mRNA expression of both aggrecan and type 2 co...

Binding of exogenous [35S]sulphate-labelled cartilage proteoglycans to cells was studied with suspension cultures of calf articular-cartilage chondrocytes. Proteoglycans interact with hyaluronic acid at the cell surface via their hyaluronic acid-binding region. The binding is time-dependent and saturable, but does not appear to be freely reversible. The bound 35S-labelled proteoglycans are loca...