Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and selectivity is, to date, however, still lacking. Here, we present a novel, simplified technique fo...

In the present study, the binding isotherms for interaction of a homologous series of n-alkyltrimethyl ammonium bromides with bovine serum albumin (BSA) have been analyzed on basis of intrinsic thermodynamic quantities. In this regards, the intrinsic Gibbs free energy of binding, AGb(i,)„ has been estimated at various surfactant concentrations and its trend of variation for both binding sets ha...

Both insufficient plasma half-life (circulation for only few hours or less) and laborious downstream purification can be bottlenecks biological drug development. We report a novel strategy the efficient gentle affinity of pharmacologically relevant proteins modified by PASylation prolonged action in vivo . previously described antibodies specific Pro/Ala-rich sequences (PAS) covering range bind...

and Yan (5) reported that they successfully separated high polarity explosives using CEC with nonporous C18-coated material and a mobile phase with a low concentration of organic modifier and SDS added to the mobile phase to increase the wettability of the particle surface. Zhang and colleagues (9) also reported that very polar compounds could be separated by mixed-packing CEC with a stationary...

Monoclonal Antibodies (mAbs) have emerged as a unique and exciting group of biological products. They represent a rapidly growing biopharmaceutical market segment with interesting and extremely useful medical applications in the treatment of numerous diseases such as cancer and immunological disorders. The monoclonal antibody purification process that is adopted should be reliable, robust and m...