Temperature-dependent free radical reactions were investigated using nitroxyl radicals as redox probes. Reactions of two types of nitroxyl radicals, TEMPOL (4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl) and carbamoyl-PROXYL (3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl), were tested in this paper. Heating a solution containing a nitroxyl radical and a reduced form of glutathione (GSH) c...

PURPOSE To characterize the possible role of transducin Gtβγ-complex in modulating the signaling properties of photoactivated rhodopsin and its lifetime in rod disc membranes and intact rods. METHODS Rhodopsin photolysis was studied using UV-visible spectroscopy and rapid scanning spectroscopy in the presence of hydroxylamine in highly purified wild-type and Gtγ-deficient mouse rod disc membr...

BACKGROUND Occupational disease linked to the paper recycling industry has not been well documented. No previously confirmed formal diagnosis of occupational asthma (OA) caused by hydroxylamine has been made. METHODS We have assessed and performed occupational assessment of eight workers involved in this industry. Two of these were later diagnosed with OA and are reported here. RESULTS Both...

An electrochemical sensor for amperometric detection of hydroxylamine was developed by electrodeposition of hybrid copper-cobalt hexacyanoferrate films on a glassy carbon electrode. The modified electrode could reduce the overpotential of the hydroxylamine oxidation by about 400 mV. This sensor exhibited a linear relationship between the response current and the hydroxylamine concentration in t...

The effect of hydroxylamine (NSC-26250) and related compounds has been examined on the growth of 11 transplanted mouse tumors, including 3 leukemias, and 1 rat tumor. By the end of therapy hydroxyurea (NSC-32065) and its 1-methyl (NSC-529064) and 1-ethyl (NSC-50840) derivatives retarded the growth of 9 tumors, and hydroxyurethan (NSC-71045; hydroxycarbamic acid ethyl ester) and N, 0-diacetyl-^-...

Drug-induced systemic lupus erythematosus arises from toxic side-effects of administration of hydralazine, isoniazid, procainamide and practolol. Hydralazine and isoniazid are nucleophilic drugs and inhibit the covalent binding reaction of complement components, C3 and C4, an effect likely to lead to deposition of immune complexes (a feature of systemic lupus erythematosus). Procainamide and pr...

The amidase from Rhodococcus erythropolis MP50 demonstrated, in the presence of hydroxylamine, acyltransferase activity and catalyzed the formation of hydroxamates from amides and hydroxylamine. The rates of acyltransferase activity of the purified amidase for the substrates acetamide, phenylacetamide, and 2-phenylpropionamide were higher than the corresponding rates for the hydrolysis reaction...

An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet P-40, bound covalently to penicillin-substituted Sepharose, and subsequently eluted from the matrix with neutral hydroxylamine, which cleaves the penicilloyl-enzyme bond. Penicillin binding-component V...