Two 36-amino acid-containing peptides (al-CBZ), isolated from rat skin and tendon collagen by cleavage of the al chain with CNBr, were subjected to enzymatic hydroxylation with collagen proline hydroxylase. The peptides dithered initially only in that the ratio of hydroxyproline to proline was considerably lower in the tendon peptide, although the imino acid content of the two peptides was the ...

Proteolytic degradation of extracellular matrix is thought to play an important role in many lung disorders. In the current study, human lung fibroblasts were cast into type I collagen gels and floated in medium containing elastase, cytomix (combination of tumor necrosis factor-alpha, interleukin-1beta, and interferon-gamma), or both. After 5 days, gel collagen content was determined by measuri...

Excessive fluoride intoxication plays an important role in the development of dental, skeletal and non-skeletal fluorosis. The aim of this study was to ascertain the toxic effect of excessive fluoride ingestion on the level of hydroxyproline and expression of type 1 collagen gene in rat bone and its amelioration by supplementation with Tamarindus indica fruit pulp extract. Forty albino rats wer...

BACKGROUND Malondialdehyde (MDA) is an oxidant that causes damage to membranes, DNA, proteins, and lipids at the cellular level. Antioxidants minimize the effects of oxidants and thus help in formation of healthy granulation tissues with higher level of hydroxyproline and total protein. This study compared the effect of limited access dressing (LAD) with conventional closed dressing biochemica...

Background: Regarding the negative effects of androgenic anabolic steroids (AASs) abuse, the long-term effect of nandrolone decanoate with/without two intensities of endurance exercise training was investigated on heart tissue and electrocardiogram (ECG) in rats. Methods: The experiment was conducted on 63 male Wistar rats, which were 4 months old. The rats were divide into groups of control (C...

In an effort to understand the role of hydroxyproline-rich glycoproteins (HRGPs) in plant cell wall structure, we studied the distribution and physical properties of PC-1-like proteins (PC-1 being the major pericarp HRGP) throughout sporophytic tissues of two maize (Zea mays L.) varieties. We determined total amounts of hydroxyproline, an indicator of HRGPs, and did tissue print and Western blo...

Phenytoin monotherapy in patients with epilepsy affects calcium metabolism and bone turnover markers leading to hypovitaminosis D, hypocalcemia, reduced bone mineral density and its imminent consequences. This study was planned to assess how early these changes may arise and to find out their correlation with phenytoin levels. In this prospective study, bone mineral density (BMD), 25-hydroxy vi...

11-78 mg./24 hr.). Free (i.e. non-peptide) hydroxylysine is usually present only in trace amounts, except during a high collagen diet. An excess of free hydroxylysine was found in Case 1 (10 0 mg./24 hr.) and Case 2 (12-3 mg./24 hr.). Case 1 also excreted an excess of citrulline (8 8 mg./24 hr.). Since no hydroxylysine was detected in the fasting bloods of controls or of patients, a renal origi...

Liakakos, D., Ikkos,. D. G., Vlachos, P., Ntalles, K., and Coulouris, Ch. (1974). Archives of Disease in Childhood, 49, 400. Effect of ascorbic acid on urinary hydroxyproline of children receiving corticosteroids. Oral administration of ascorbic acid, 0 * 5 g every 8 hours for a period of 4 days, had no effect on the urinary hydroxyproline excretion of 7 healthy children aged 8 to 14 years. Whe...