Alpha toxin derived from Clostridium perfringens was used for diagnosis of Streptococcus agalactiae (St.agalactiae) by rapid and spot CAMP tests. The selected Cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by DEAE cellulose chromatography. The efficacy of both purified and crude α-toxins were examined by rapid...