SYNOPSIS Immunofluorescent serum IgM and/or a significant level of IgA antibody was detected in 87 % of 39 cases of current or recent influenza A infection from two to 84 days after the onset of illness. Secondary IgM staining occurred in 5% of sera and a significant correlation was found between complement-fixing and class-specific antibodies. It was estimated that the immunofluorescent test c...

This study estimated the sensitivity and specificity of the rK39 strip test compared with the immunofluorescent antibody test and microscopy of bone marrow aspirate smears (the gold standard) in 47 children with suspected visceral leishmaniasis. A control group of children with other diagnoses (tuberculosis, toxoplasmosis, systemic lupus erythematosus, malaria or cutaneous leishmaniasis) were a...

Significant advances in the techniques available for rabies diagnosis have taken place over the past thirty years. The traditional method of histological examination for Negri bodies in the brain sections has been largely replaced by immunofluorescent staining methods for the detection of nucleocapsid antigen in brain smears (fluorescent antibody test: FAT) (Dean and Abelseth, 1973). Neverthele...

A 20-year-old man presented with elevated serum creatinine level 3 months after kidney transplantation. Cytomegalovirus (CMV) pp65 antigen was detected by indirect immunofluorescent method. Figure A shows contaminated cells in phosphorous green (× 400). Kidney needle biopsy was performed and tubulointerstitial nephritis with CMV cytopathic effects was observed on pathologic examination. No obvi...

An enzyme immunoassay (EIA) was developed for detecting antibody to herpes simplex virus, and the results were compared with those of complement fixation, indirect immunofluorescent-antibody, and plaque reduction neutralization tests. Test sera showed very little nonspecific reactivity even at a starting dilution as low as 1:10. EIA results showed excellent correlation with results obtained by ...

A low-power laser-UV microbeam of wavelength 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphflse or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts ofDNA treated with far UV ligh t. The results show that antibodies specific for UVirradiated DNA can be used fo...

Immunofluorescent serum IgM and/or a significant level of IgA antibody was detected in 87% of 39 cases of current or recent influenza A infection from two to 84 days after the onset of illness. Secondary IgM staining occurred in 5% of sera and a significant correlation was found between complement-fixing and class-specific antibodies. It was estimated that the immunofluorescent test could be di...

Circulating antigliadin antibody has been described in patients with gluten enteropathy although the prevalence varies in different studies. It has been suggested that the investigation for antigliadin antibody might be useful as a screening test. The object of the present study was to evaluate two different techniques for assaying these antibodies - an indirect immunofluorescent method and an ...

alpha2-Macroglobulin (alpha2M) has been identified on the luminal surface of endothelial cells in sections of normal human arteries, veins, and lymphatics by the indirect immunofluorescent technique. The specificity of the immunofluorescent reaction was confirmed by immunoabsorption studies. Prior absorption of the anti-alpha2M antiserum by purified alpha2M at equivalence completely inhibited e...

Electron microscopic and immunofluorescent studies are reported in a patient with idiopathic pulmonary hemosiderosis, suggesting that idiopathic pulmonary hemosiderosis is distinct from other pulmonary hemorrhagic syndromes associated with antibasement membrane antibody or immune complex mediated diseases. The ultrastructural studies showed evidence of nonspecific lung injury and indirect evide...