background: human granulocyte colony-stimulating factor (hg-csf) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other a...

objective: the incidence of breast cancer is approximately one million which makes this cancer one of the most common among women worldwide. breast cancer comprises 7% of the total death rate caused by cancers. several strategies that use tumor-associated antigen (taa) vaccination and early detection of breast cancer are clinically being developed. breast cancer is caused by increased over expr...

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

vp2 gene coding region of a vaccinal strain (d78) of infectious bursal disease virus (ibdv) was clonedin a eukaryotic expression vector, psec tag2a. the gene was placed downstream of ig κ chain leadersequence, under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. theconstruct psec tag2a-vp2 was transfected in cos-7 cell line and the expression and secretion of...

classical lbp such as complexity and high dimensions of feature vectors that make it necessary to apply dimension reduction processes. in this paper, we introduce an improved lbp algorithm to solve these problems that utilizes fast pca algorithm for reduction of vector dimensions of extracted features. in other words, proffer method (fast pca+lbp) is an improved lbp algorithm that is extracted ...

We describe the development of a novel positive-selection vector, RHP-AmpS, that is suitable for seamless cloning and high-level protein expression in Escherichia coli. In this vector, beta-lactamase (Bla) was rendered nonfunctional by replacing the codon for the C-terminal amino acid of the beta-lactamase gene (bla) with a stop codon. Insertion of a target gene in the correct orientation (tail...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

glioblastoma is the most common and the most lethal primary brain cancer. this malignancy is highly locally invasive, rarely metastatic and resistant to current therapies. little is known about the distinct molecular biology of glioblastoma multiforme (gbm) in terms of initiation and progression. so far, several molecular mechanisms have been suggested to implicate in gbm development. homeodoma...