Objective(s): The aim of this work was to prepare and characterize magnetic nanoparticles (MNPs) as theranostic system to act simultaneously as drug carrier and MRI contrast agent. Chitosan-coated MNPs (CMNPs) were prepared and loaded with silymarin. Silymarin-loaded CMNPs were characterized with various techniques and their potential as MRI contrast agent was also evaluated. Materials and Meth...

INTRODUCTION Rat mesenchymal stem cells (MSCs) represent a small portion of the cells in the stromal compartment of bone marrow and have the potential to differentiate into new blood vessel and other tissues. MSCs transplantation in tissues from critical limb ischemia model in rat may represent a therapeutic applications of vascular regeneration. AIM The aim of this study was to isolate with ...

Multicellular spheroids are an established system for three-dimensional cell culture. Spheroids are typically generated using hanging drop or non-adherent culture; however, an emerging technique is to use magnetic levitation. Herein, mesenchymal stem cell spheroids were generated using magnetic nanoparticles and subsequently cultured within a type I collagen gel, with a view towards developing ...

Objective(s): Exosomes are endogenous nanovesicles act as intercellular communication tools which have been considered to utilize as drug delivery systems. As transporting therapeutic molecules into brain has obstacles, preparing exosomes which have the potential to pass through its barriers is great challenge. Methods: Exosomes isolated from cell culture media of U87 glioblastoma ...

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of ...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

Background: In the present study, a tissue engineered silk fibroin (SF) scaffold containing simvastatin-loaded silk fibroin nanoparticles (SFNPs) were used to stimulate the regeneration of the defected bone. Methods: At first, the porous SF scaffold was prepared using freeze-drying. Then simvastatin-loaded SFNPs were made by dissolvation method and embedded in the SF scaffold. Afterwards, the ...

The aim of this study was to address one of the major challenges of the actual era of nanomedicine namely, the bioavailability of poorly water soluble drugs such as Silymarin. We developed new, biodegradable, and biocompatible nanosized shuttles for Silymarin targeted delivery in colon-cancer cells. The design of these 100 nm sized carrier nanoparticles was based on natural polymers and their b...

Background & Aim: Effects of ultra violet spectrum on plants due to their obligatory requirement for sunlight for survival are unavoidable. Direct effects of UV on plant growth and development are generally negative and usually they use many different defense mechanisms to acclimate and protect themselves. Material & Methods: The present research for the first time was conducted to investigate...