The diagnosis of an allergy in a patient can be very complicated. Over the years a number of methods have been developed for the detection of the small amounts of specific-IgE (sIgE) in human serum. Selecting the appropriate allergy test can be difficult and it should be in context of a patient’s clinical presentation. The relevance of a test may also vary according to the patient’s age, allerg...

The in vivo imaging of mice makes it possible to analyze disease progress non-invasively through reporter gene expression. As the removal of hair improves the accuracy of in vivo imaging, gene-modified mice with a reporter gene are often crossed with Hos:HR-1 mutant mice homozygous for the spontaneous Hrhr mutation that exhibit a hair loss phenotype. However, it is time consuming to produce mic...

The vertebrate retina develops rapidly from a proliferative neuroepithelium into a highly ordered laminated structure, with five distinct neuronal cell types. Like all neurons, these cells need to polarize in appropriate orientations order integrate their neuritic connections efficiently into functional networks. Its relative simplicity, amenability to in vivo imaging and experimental manipulat...

We demonstrate a novel imaging technique, named short-lag spatial coherence (SLSC) imaging, which uses short distance (or lag) values of the coherence function of backscattered ultrasound to create images. Simulations using Field II are used to demonstrate the detection of lesions of varying sizes and contrasts with and without acoustical clutter in the backscattered data. B-mode and SLSC imagi...

Bouldin et al. 1 described an improved version of the zebrafish Fucci system, which permits in vivo imaging of the cell cycle state using fluorescent proteins. Whereas the original system required crossing two different fish to produce embryos expressing both reporter transgenes, the new system, called Dual Fucci, expresses both reporters from a single transgene (Fig. 1), thus permitting crosse...

Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the genomes of diverse organisms. These advances expand the potential of in vivo imaging experiments and facilitate experimentation with new, bright, photostable fluorescent prot...

A fast chemical-shift imaging using the gradient-echo technique is proposed for the separation of the water and fat in human in vivo imaging. This technique also incorporates magnetic field inhomogeneity correction in the fast chemical-shift imaging. Experimental results of the proposed imaging technique demonstrate that the method would be useful for quick in vivo water-fat separation imaging ...

In vivo bioluminescent imaging (BLI) permits the visualization of engineered bioluminescence from living cells and tissues to provide a unique perspective toward the understanding of biological processes as they occur within the framework of an authentic in vivo environment. The toolbox of in vivo BLI includes an inventory of luciferase compounds capable of generating bioluminescent light signa...

Far-red fluorescent proteins are critical for in vivo imaging applications, but the relative importance of structure versus dynamics in generating large Stokes-shifted emission is unclear. The unusually red-shifted emission of TagRFP675, a derivative of mKate, has been attributed to the multiple hydrogen bonds with the chromophore N-acylimine carbonyl. We characterized TagRFP675 and point mutan...

The concept of a multispectral confocal microscope for in vivo imaging is introduced. To demonstrate the concept we modified a slit-scan fluorescence confocal microendoscope incorporating a fiber-optic catheter for in vivo imaging to record multispectral images. The system was designed to examine cellular structures during optical biopsy and to exploit the diagnostic information contained withi...