While it is generally recognized that misfolding of specific proteins can cause late-onset disease, the contribution of protein aggregation to the normal aging process is less well understood. To address this issue, a mass spectrometry-based proteomic analysis was performed to identify proteins that adopt sodium dodecyl sulfate (SDS)-insoluble conformations during aging in Caenorhabditis elegan...

Cellulose acetate fibers are commonly made by a dry-spinning process, in which cellulose acetate is firstly dissolved in acetone and then the cellulose acetate acetone solution is extruded through spinneret orifices that have extremely small diameters. Any insoluble substances in acetone solutions could cause disruptions in fiber extrusion, and are not tolerated. In this work, acetone-insoluble...

vitro digestibility tended to be greater with ammoniation. In the second trial, very high levels of ammonia, urea or glucose were added. Levels of insoluble protein were much higher in this trial than the other. This reflects the high variability of corn silage. Recovery of nitrogen added as ammonia averaged 82 percent while all of the nitrogen added as urea was recovered. The amounts of insolu...

Acetic acid-soluble and insoluble proteins were isolated from doughs mixed in the presence of N-ethylmaleimide (NEMI). Reducedpyridylethylated acetic acid-insoluble protein (AF) and reducedpyridylethylated acetic acid-soluble protein (BF) were sequentially fractionated into an acetic acid-insoluble fraction (F-0), an acidic 70% ethanol-insoluble fraction (F-I), a neutral 70% ethanol-insoluble f...

A study of the precipitability by the appropriate antisera of 34 different haptens, containing from one to six reactive groups, leads to the conclusion that the possibility of framework ("lattice") formation is neither necessary nor sufficient for specific precipitation, but that instead precipitation depends upon the reduction, by mutual neutralization of polar groups of antibody and antigen (...

Water-insoluble enzyme derivatives possessing specific catalytic activity may provide a useful tool in biochemical studies, since they can readily be removed from the reaction mixture and can be used for the preparation of columns with enzymatic activity. The conversion of several enzymes into the corresponding water-insoluble derivatives by their binding via covalent bonds to insoluble carrier...

El objetivo de este estudio fue evaluar la composición química seis gramíneas forrajeras para el trópico húmedo y seco mexicano: Andropogon gayanus, gerardii, Cenchrus ciliaris, Chloris gayana, Megathyrsus maximus Urochloa brizantha. Las muestras se colectaron en noviembre 2019, Tonaya, Jalisco, México. Se determinaron: materia seca, cenizas, proteína cruda (total e insoluble), extracto etéreo,...

Paenibacillus sp. W-61 is capable of utilizing water-insoluble xylan for carbon and energy sources and has three xylanase genes, xyn1, xyn3, and xyn5. Xyn1, Xyn3, and Xyn5 are extracellular enzymes of the glycoside hydrolase (GH) families 11, 30, and 10, respectively. Xyn5 contains several domains including those of carbohydrate-binding modules (CBMs) similar to a surface-layer homologous (SLH)...