Herein, the interaction of hen egg white lysozyme (HEWL) with iron nanoparticle (Fe NP) was investigated by spectroscopic and docking studies. The zeta potential analysis revealed that addition of Fe NP (6.45±1.03 mV) to HEWL (8.57±0.54 mV) can cause to greater charge distribution of nanoparticle-protein system (17.33±1.84 mV). In addition, dynamic light scattering (DLS) study revealed that add...

Using helium cluster-isolation spectroscopy we have prepared and characterized the lowest quartet state (14A2) of the sodium trimer. Upon excitation to the 24E0 electronic state, laser induced fluorescence excitation and emission spectra have been obtained. With the aid of ab initio calculations, the spectra have been vibronically assigned and new insights on the nonadditivity of van der Waals ...

The fluorescence properties of porphyrin were compared in protoporphyrin-protoheme hybrid hemoglobins (Hb) in which the protohemes (Fe) in either the aor P subunits were substituted with protoporphyrin IX, a(P),p(Fe),-Hb and a(Fe),p(P),-Hb, respectively. The fluorescence lifetimes of the porphyrin in these hybrid derivatives are shorter than for the tetra-substituted protoporphyrin Hb, indicati...

This paper describes a new method of analysis of fluorescence images containing a mixture of fluorophores. Parametric maps showing the relative quantity and distribution of each individual fluorophore are generated using a predictive model of image formation. Error analysis applied to the model supplies objective criteria for choosing the optimal filters for image acquisition in the regions in ...

Under selective excitation of the P0 level of the Pr3+ ion in YPO4, the emission spectra and fluorescence decay curves were measured at different concentrations and temperatures. The origin of the spectral line located at 16 318 cm−1 (612.8 nm) was discussed and attributed to the D2 → H4 transition. The process of concentration quenching for the D2 state was also studied. Using the Inokuti–Hira...

We report new measurements of fluorescence excitation spectra and single rovibronic level fluorescence lifetimes for ð0; t2; 0Þ ð0; 0; 0Þ bands with t2 1⁄4 0–6 in the HCF ~ AA 00 ~ X1A0 system. Due primarily to the Renner–Teller interaction, upper state levels with Ka P 1 display an obvious lengthening of lifetime with increasing energy which is strongly correlated with the presence of vibronic...

BACKGROUND AND OBJECTIVE Fluorescence spectroscopy has been shown to provide information useful in the detection of cervical dysplasia. The goal of this study was to determine if substances found on the cervix such as acetic acid, mucus, and vaginal medications can influence the fluorescence in the spectral region useful for discriminating normal cervical tissue from abnormal tissue. STUDY DE...

1,11-Didechloro-6-methyl-4'-O-demethyl rebeccamycin (JDC-108), a rebeccamycin analog possessing potent anti-tumor activities, was prepared via a concise one-pot strategy in good yield. The interaction between JDC-108 and human serum albumin (HSA) was studied by spectroscopic methods including fluorescence spectroscopy, UV-vis absorption spectrum, and molecular modeling. The quenching mechanism ...

The influence that urea has on the conformation of water-soluble globular protein, bovine serum albumin (BSA), exposed directly to the aqueous solution as compared to the condition where the macromolecule is confined in the Aerosol-OT (AOT sodium bis-2-ethylhexyl sulfosuccinate)/n-hexane/water reverse micelle (RM) is addressed. Small angle X-ray scattering (SAXS), tryptophan (Trp) fluorescence ...