for evaluation of citric acid and citrate effects on bovine epididymal protoplasmic droplets, fifty bovine testes were collected in the october 2007 till june 2008 from urmia slaughterhouse and transported to the laboratory in a cool container filled with 5 °c ice pack. caudal epididymis was incised and sperm cells were put into petri dishes containing hams f10 media with 10% fetal calf serum (...

Mouse sperm-egg fusion was examined using two-photon and confocal microscopy. A delay of several minutes occurred between the first observable event of fusion (which was the diffusion of Ca2+-sensitive dyes from egg into sperm) and any change in egg cytoplasmic Ca2+. When indo-1 dextran was used to obtain ratiometric two-photon images, there was no detectable local increase in egg cytoplasmic C...

Background: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. Methods: Oocytes were obtained from nine infertile women wit...

Background: Comparison of obstetric and neonatal outcomes of twin pregnancies conceived by assisted reproduction technology (ART) with those of spontaneously conceived (SC). Materials and Methods: In this prospective cohort study, all dichorionic twin pregnancies following fresh IVF/ intra-cytoplasmic sperm injection (ICSI) or ICSI cycles (n=320) and (SC) (n=170) in nulliparous women in Royan I...

Background Implantation failure is the most important cause of recurrent in vitro fertilization/intra cytoplasmic sperm injection (IVF/ICSI) failure. Several reports suggest that intercourse during the peritransfer period might improve pregnancy rates. This study is designed to determine whether intercourse during the peritransfer period will improve pregnancy and implantation rates in patients...

Submit Manuscript | http://medcraveonline.com Abbreviations: WHO: World Health Organization; ROS: Reactive Oxygen Species; PS: Phosphatidylserine; EPS: Externalization of Phosphatidylserine; ART: Assisted Reproduction Techniques; IUI: Intra-Uterine Insemination; IVF: In Vitro Fertilization; ICSI: Intracytoplasmic Sperm Injection; PICSI: Physiologic Intracytoplasmic Sperm Injection; SU: Swim-Up;...

Background: Short time preservation of semen is common in animals (e.g. horse or pig etc…) which their sperm in known as freezing sensitive sperm. It also may use for short transportation of other species to be cryopreserved or use in AI or IVF programs. The aim of the present study was to evaluate effects of duration of cool storage on goat epididymal sperm. Materials and Methods: Total number...

Abstract Study question How should men with globozoospermia be managed in Assisted Reproductive Technologies (ART)? Summary answer IMSI (intracytoplasmic morphologically selected sperm injection), without oocyte activators (OA) allows to obtain a live birth, even when there is mutation of DPY19L2 gene. What known already Globozoospermia rare syndrome found less than 0.1% the infertile populatio...