The intestinal mucosa forms the first line of defense against infections mediated by enteric pathogens such as salmonellae. Here we exploited intestinal "organoids" (iHOs) generated from human induced pluripotent stem cells (hIPSCs) to explore the interaction of Salmonella enterica serovar Typhimurium with iHOs. Imaging and RNA sequencing were used to analyze these interactions, and clear chang...

An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is poorly understood. We have analyzed the expression and localization of PrgJ a...

Background: Salmonella is an important foodborne pathogen that causes diarrhea in humans worldwide. Objectives: This study aimed to determine the serotype distribution, antibiotic-resistant genes, and pathogenicity island (SPI) genes of clinical isolates Weifang. Methods: A total 111 strains were collected from Weifang People’s Hospital between 2018 2020 subjected serotyping using Kauffmann-Whi...

objective(s): the aim of this study was to investigate the methylation status and mrna expression levels of p15, death-associated protein kinase (dapk), and suppressor of cytokine signaling-1 (socs1) genes in multiple myeloma (mm). materials and methods: the bone marrow samples of 54 mm patients were collected and the methylation status of the p15, dapk, and socs1 gene promoter regions was dete...

conclusions some results of the correlation coefficients are not the same with visualization. the reason may be due to the small number of data. materials and methods in the foundation-application study, we constructed two-way gene networks using nonparametric methods, such as spearman’s rank correlation coefficient and blomqvist’s measure, and compared them with pearson’s correlation coefficie...

An auxotrophic mutant of nontyphoidal Salmonella (NTS) strain (Salmonella Oslo) was phenotypically characterized in this study. The characterization based on phenotype, morphology, motility, biofilm forming ability, growth kinetics, etc. phenotypic results from the above experiments determined that showed variation characters wild-type strain. Subsequently, and NTS were subjected to epithelial ...

slow growing, primary pleural neoplasm unrelated to asbestos exposure, and accounts for fewer than 5% of pleural tumors (1). It occurs in both sexes and in all age groups, but predominantly affects those in the fifth to seventh decades of life. England et al. (2) found that 60% of these tumors were benign and 40% were malignant, as determined microscopically on the basis of cellularity, pleomor...