The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behi...

reteplase is a segment of tissue plasminogen activator used for the removal of thrombi in blood vessels. in the present study the cloned reteplase gene was used for its expression in competent e. coli. the  recombinant plasmid, pet15b/reteplase (rpet-bl21), was transformed into competent e.coli strain bl21 (de3) cells. overnight culture of the transformed bacteria was induced by the addition of...

Mục tiêu: Xây dựng quy trình biểu hiện enzym tái tổ hợp Br512 và ứng dụng phát Human Papillomavirus type 18 bằng phương pháp khuếch đại đẳng nhiệt qua trung gian vòng lặp (LAMP). Vật liệu pháp: Plasmid pKAR2-Br512 mã hoá enzyme BR512 được biến nạp vào tế bào E.coli BL21(DE3). Enzyme từ đó chất cảm đặc hiệu (IPTG) sau tinh sạch sắc ký ái lực. Đánh giá tính của tự sản xuất, so sánh với Bst 2.0 (B...

Staphylokinase, a 163 amino aci d bacterial protein from Staphylococcus aureus is reported to have a therapeutic function and used as a potential alternative for the available clot dissolving drugs and some countries proved to be a potent alternative over the available clot dissolving drugs. In the present study, sak gene is isolated from a lysogenically converted S.aureus and expressed in non-...

Malaria is still an essential epidemiological disease worldwide, including in Indonesia. Several approaches are performed to control the disease, as well vaccine development. The Cysteine-rich interdomain region α of Plasmodium falciparum erythrocyte membrane protein 1 (CIDRα-PfEMP1) a pivotal domain malaria pathogenesis make it candidate. development using recombinant technology. Recombinant p...

Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector. The optimization of the activin A production in E. coli was done by using the response surface methodology (RSM). At this stage, the effect of IPTG and la...

The poultry industry in Vietnam is vulnerable to many viral diseases. Since antibiotics and vaccination provide inadequate protection, it will be beneficial have alternative immunostimulants boost non-specific immunity poultry. Chicken interferon-alpha (ChIFNα) has been described previously with antiviral activity against pathogens. Therefore, the purpose of this study clone express recombinant...

The majority of therapy methods include downsides and limits. As a result, many researchers are focused on developing effective remedies. Therapeutic peptides, like proteins antibodies, potential class medications that have number advantages over traditional pharmaceuticals. Williamson marianensis-produced cholesterol oxidase has been demonstrated to medicinal value. Using PCR primers specific ...

objective(s): streptokinase (ska) is an antigenic protein which is secreted by streptococcus pyogenes. streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. in the present study, recombinant streptokinase from s. pyogenes was produced and showed that recombinant ska protein was recognized by infected human sera using western blot analysi...