A complex deposition procedure of the hydrogel layer of modified poly(2hydroxyethyl methacrylate) (polyHEMA) covalently linked to the silicon nitride surface and covering only the gate area of the ISFET, was optimized for photolithographic technology, using standard silicon wafers of 3” diameter. The influence of hydrogel composition and layer thickness on the sensors’ parameters was investigat...

This paper describes the development of ISFETs in an historical setting, but is not limited to that. Based on the development regarding the theory, the technology, the instrumentation and the experience with many specific applications, also future projects are defined, such as concerning cell acidification, REFET biasing and a complete new range of FET sensors based on local pressure induction ...

Ion-sensitive field-effect transistors (ISFETs) in combination with unmodified complementary metal oxide semiconductors present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive specific detection of two circulating mRNA markers prostate cancer, androgen receptor TMPRSS2-ERG fusion using target-specific loop-mediated isothermal amplification ...

For the visualization of chemical species in the solution, markers and labels such as fluorescent dyes are often utilized. For certain applications in biology, however, toxicity of such dyes may be a problem, especially in the case of cells and tissues in culture. In this study, a label-free method of ion imaging based on a semiconductor device is developed. Chemical sensors based on semiconduc...