‘California Blackeye 77’ (CB77) (Reg. no. CV-344, PI 698656) is a blackeye-type cowpea [Vigna unguiculata (L.) Walp] cultivar developed by the University of California, Riverside. CB77 distinguished from industry standard cultivars, 46’ (CB46) and 5’ (CB5), its ability to resist infestation caused aphid (Aphis craccivora Koch), destructive sap-sucking insect pest prevalent in Central Valley Cal...

Cold atmospheric plasma (CAP) has emerged as a highly selective anticancer agent, most recently in the form of plasma-activated medium (PAM). Since epithelial–mesenchymal transition (EMT) been implicated resistance to various cancer therapies, we assessed whether EMT status is associated with PAM response. Mesenchymal breast cell lines, well mesenchymal variant an isogenic EMT/MET human system ...

Hybrid sterility is a reproductive barrier that prevents gene flow between species. In Oryza species, some hybrid loci, which are classified as gamete eliminators, cause pollen and seed sex-independent transmission ratio distortion (siTRD) in hybrids. However, the molecular basis of siTRD has not been fully characterized because lacking information on causative genes. Here, we analyze one S2, w...

We have generated a murine embryonic stem cell line constitutively expressing L1 at all stages of neural differentiation to investigate the effects of L1 overexpression on stem cell proliferation, migration, differentiation, cell death, and ability to influence drug-induced rotation behavior in an animal model of Huntington's disease. L1-transfected cells showed decreased cell proliferation in ...

The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae) is a significant soybean pest in the north central United States. Insecticidal seed treatments and host plant resistance are two commercially available management tools. Here we investigate the efficacy of both management tools throughout the season. Soybean lines containing the soybean aphid resistance genes Rag1, Rag2, or both...

To gain new insights into the mechanism of soybean (Glycine max) resistance to the soybean cyst nematode (Heterodera glycines), we compared gene expression profiles of developing syncytia in soybean near-isogenic lines differing at Rhg1 (for resistance to Heterodera glycines), a major quantitative trait locus for resistance, by coupling laser capture microdissection with microarray analysis. Ge...

Cisplatin resistance is one of the major reasons leading to the high death rate of ovarian cancer. Methyl-Capture sequencing (MethylCap-seq), which combines precipitation of methylated DNA by recombinant methyl-CpG binding domain of MBD2 protein with NGS, global and unbiased analysis of global DNA methylation patterns. We applied MethylCap-seq to analyze genome-wide DNA methylation profile of c...

BACKGROUND The use of cytoplasmic male sterility (CMS) in F1 hybrid seed production of chili pepper is increasingly popular. However, the molecular mechanisms of cytoplasmic male sterility and fertility restoration remain poorly understood due to limited transcriptomic and genomic data. Therefore, we analyzed the difference between a CMS line 121A and its near-isogenic restorer line 121C in tra...