transferrin acts as a vehicle for iron transportation between the absorption, storage and utilization sites in the body and, besides iron, its two highaffinity iron-binding site are capable of binding to 29 other elements. in physiological and pathophysiological conditions, transferrin’s properties are different and close attention to these properties is required. for further investigation for ...

Articular cartilage, the load-bearing tissue of the joint, has limited repair and regeneration ability. The scarcity of treatment modalities for large chondral defects has motivated researchers to engineer cartilage tissue constructs that can meet the functional demands of this tissue in vivo. Cartilage tissue engineering requires 3 components: cells, scaffold, and environment. ...

in west africa and nigeria in particular, many virgin plants are still waiting to be evaluated for their medicinal importance. claims of plants with folk medicinal applications need to be evaluated and verified. gomphrena celosioides (family – amaranthaceae) is a weed grown in lawns and the biological activity of the extract had earlier been established. in the present study, the plant was coll...

background and objective : salmonellosis can be acquired through consumption of infected raw or undercooked eggs. the aim of this study was isolation and identification of salmonella spp from the eggshells and the egg contents samples of tabriz retails. methods : a total number of 150 samples of eggs were analyzed for the presence of salmonella spp. using conventional culture method and multipl...

background kelussia odoratissima mozaff. is one of the newest genera of umbelliferae which is represented by only one species. this sweet-smelling, self-growing monotypic medicinal plant is endemic to a restricted area in west of iran and is locally called karafse-koohi. the aerial parts of the plant are commonly used as a popular garnish and a sedative medicinal plant. there are several report...

BACKGROUND RNA from sorted cell populations is crucial in many instances. We therefore compared four current protocols for RNA isolation, with regard to mRNA yield and purity. Moreover, we examined the effects on RNA recovery caused by different storage reagents. METHODS Small populations of K562 cells or PMBC were sorted into the lysing reagent and subjected to RNA extraction, employing eith...

The transformation-associated recombination (TAR) cloning technique allows selective and accurate isolation of chromosomal regions and genes from complex genomes. The technique is based on in vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or hooks, to the gene of interest. The recombination occurs during transformation of yeast spheroplasts that ...

Since 1980, RP chromatography has been the principal analytical technique used for cephalosporins. This technology offers selectivity, accuracy, and ease of use. Most of the methods rely on protein precipitation and, to a lesser extent, solid-phase isolation or extraction procedures. The proper selection of a method depends on the analytical constraints imposed by the overall objective of the s...

the rhizome of anemarrhena asphodeloides is used as food and traditional chinese medicine for its hypoglycemic effect. the aim of this study was to investigate the isolation, purification and hypoglycemic activity of anemaran as the active component. the influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. the opti...

background: there are eight taxus species and two hybrids in the world and taxus baccata l. (european yew) is the single representative in iran. until now, a large number of taxoids possessing different skeleton systems, as well as lignans, flavonoids, steroids and sugar derivatives have been isolated from various taxus species. taxoids are highly oxygenated diterpenes isolated from different s...