This paper describes the synthesis and application of alginate-chitosan-cyclodextrin micro- and nanoparticulate systems loaded with isoniazid (INH) and isoconazole nitrate (ISN) as antimycobacterial compounds. Preparation and morphology of the obtained particles, as well as antimycobacterial activity data of the obtained systems are presented. Docking of isoconazole into the active site of enoy...

Prompt detection of drug resistance in Mycobacterium tuberculosis is essential for effective control of tuberculosis (TB). We developed a Multi-PCR-SSCP method that detects more than 80% commonly observed isoniazid (INH) and rifampin (RIF) resistance M. tuberculosis in a single assay. The usefulness of the newly developed method was evaluated with 116 clinical isolates of M. tuberculosis. Disti...

PURPOSE To compare the rapid colorimetric nitrate reductase based antibiotic susceptibility (CONRAS) test performed on Mycobacterium tuberculosis isolates with the conventional method i.e, the proportion method. METHODS One hundred clinical isolates of M. tuberculosis were tested for susceptibility to isoniazid (INH) and rifampicin (RIF) by the conventional proportion method and CONRAS in Mid...

Eight new complexes of Cu(II), Co(II), Ni(II) and Zn(II) with isonicotinic acid hydrazide (isoniazid, (INH)) and isonicotinic acid (1-naphthylmethylene)hydrazide (INNMH), having the formula of the type [M(INH)(ac)2] or [M(INNMH)(ac)2] (M = Co(II), Ni(II) and Zn(II)) and [Cu(INH)(ac)2]2, [Cu(INNMH)(ac)2]2, were synthesized and characterized. All complexes were characterized based on elemental an...

Minimal inhibitory concentrations of rifampin for different species of mycobacteria were determined in 7H-10 agar medium and Lowenstein-Jensen egg medium. When rifampin was incorporated into egg medium, approximately 90% of its activity was lost. The stability of rifampin was tested during storage at different temperatures and concentrations. When tested in agar medium, a combination of isoniaz...

BACKGROUND The largest outbreak of isoniazid-resistant (INH-R) Mycobacterium tuberculosis in Western Europe is centred in North London, with over 400 cases diagnosed since 1995. In the current study, we evaluated the genetic variation in a subset of clinical samples from the outbreak with the hypothesis that these isolates have unique biological characteristics that have served to prolong the o...

Background: Tuberculosis (TB) may be increased with biologic therapy (BT). Diagnosis and treatment of latent TB infection (LTBI) is the best strategy to prevent TB. In Rheumatic Immune-Mediated Diseases (R-IMID) LTBI, chemoprophylaxis must used before BT. The drugs more frequently are Isoniazid (INH), Rifampicin (RIF) Fluoroquinolones (FQ). These associated side-effects, especially hepatotoxici...

The newer rifamycin, rifalazil (RLZ) (previously known as KRM-1648), has been shown in prior experiments to be a highly potent drug against Mycobacterium tuberculosis. In this report, we studied the efficacy of RLZ in combination with pyrazinamide (PZA) and ethambutol (EMB) in a long-term in vivo experiment and compared their activity with the isoniazid (INH)-rifampin (RIF) combination which is...

The objective : to evalute effectiveness in clinical trials of the new kit reagents AmpliTest® MBT-Resist-I based on real-time PCR (RT-PCR) versus similar kits reagent registered Russia, identify genetic markers M. tuberculosis complex (MTB) resistance rifampicin (RIF) and isoniazid (INH) human biological samples MTB cultures. Subjects Methods . 200 DNA were studied, they had been obtained from...

Isoniazid is one of the widely used first-line drugs in the treatment of tuberculosis for many years but the mechanism of the action of the drugs is still not clear. Herein, we report the synthesis, QSAR model study and biological effects of esters and thioester derivative of isonicotinic acid (INA) on different strains of Mycobacterium tuberculosis in an attempt to establish the mode of action...