We present a method of rapid isothermal amplification of DNA without initial heat denaturation of the template, and methods and probes for (a) real-time fluorescence detection and (b) lateral flow detection of amplicons. Isothermal strand displacement amplification (iSDA) can achieve >10(9)-fold amplification of the target sequence in <20 minutes at 49 °C, which makes it one of the fastest exis...

Nickel oxide thin films have been widely studied as an optical active layer in electrochromic devices. It is well known for these films that thermal treatment is the key factor which determines the electrochromic effect during potential cycling. Therefore, it is extremely important to perform thermal analysis on the thin films rather than on the corresponding xerogels. In this article, the ther...

A pathway of post-weld heat treatment (PWHT) to eliminate affected zone (HAZ) softening and improve the strength welded joints DP980 steel was developed. It is found that high temperature isothermal transition at 750–950 °C followed by water-quenching significantly influences fracture location changing microstructures joint. The block martensite distributed discretely in island ferrite after PW...

the effect of t6 heat treatment on the microstructure and wear behavior of al-332 piston alloy containing 1.2wt% iron was investigated. the results showed that the t6 heat treatment changed the morphology of acicular eutectic si particles to a more spherical form. the heat treatment also reduced the average length and blunted the needle-like ?- phase intermetallics through dissolution and fragm...

In the present study, isothermal decomposition of austenite to bainite in 1.0 wt% carbon, 0.21% silicon steel during partitioning step a quenching and (Q&amp;P) heat treatment has been investigated dilatometer temperature range 200 350 °C compared conventional austempering treatment. The transformation was shortened by about 75% presence pre-existing martensite (QP). kinetics is described well-...

This paper presents an experimental study on simultaneously measuring the elastic modulus and residual stress of a thermal barrier coating (TBC) after different isothermal heat treatments. The TBCs were theoretically analyzed based composite beam bending theory. Thereafter, setup was established combining 3D digital image correlation method with test to obtain curvature changes in TBC sample. F...