نتایج جستجو برای: keywords aptamer

تعداد نتایج: 1982596  

2010
Chunyan Yao Tangyou Zhu Yongzhi Qi Yuhui Zhao Han Xia Weiling Fu

The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performanc...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2013
Georg Mahlknecht Ruth Maron Maicol Mancini Bilha Schechter Michael Sela Yosef Yarden

Aptamers, oligonucleotides able to avidly bind cellular targets, are emerging as promising therapeutic agents, analogous to monoclonal antibodies. We selected from a DNA library an aptamer specifically recognizing human epidermal growth factor receptor 2 (ErbB-2/HER2), a receptor tyrosine kinase, which is overexpressed in a variety of human cancers, including breast and gastric tumors. Treatmen...

Journal: :The Analyst 2014
Thangavel Lakshmipriya Yukichi Horiguchi Yukio Nagasaki

In order to detect an extremely low amount of human coagulation factor IX (FIX), poly(ethylene glycol) (PEG)/aptamer co-immobilized surface was constructed using original PEG-polyamine surface modification agents on surface plasmon resonance (SPR) sensor chip. Initially, a gold (Au) sensor chip of SPR was modified using poly(ethylene glycol)-b-poly[2-(N,N-dimethylamino)ethyl methacrylate] (PEG-...

2016
Agnivo Gosai Xiao Ma Ganesh Balasubramanian Pranav Shrotriya

The binding/unbinding of the human thrombin and its 15-mer single stranded DNA aptamer, under the application of external stimulus in the form of electrostatic potential/electric field, is investigated by a combination of continuum analysis and atomistic molecular dynamics simulation. In agreement with the experiments that demonstrate the influence of electrostatic potential on the thrombin/apt...

2015
Lauren R. Schoukroun-Barnes Ryan J. White

The relative ease of predicting the secondary structure of nucleic acid sequences lends itself to the design of sequences to perform desired functions. Here, we combine the utility of nucleic acid aptamers with predictable control over the secondary structure to rationally design sequences with controlled affinity towards a target analyte when employed as the recognition element in an electroch...

Journal: :Cancer immunology research 2015
Eli Gilboa Alexey Berezhnoy Brett Schrand

Modulating the function of immune receptors with antibodies is ushering in a new era in cancer immunotherapy. With the notable exception of PD-1 blockade used as monotherapy, immune modulation can be associated with significant toxicities that are expected to escalate with the development of increasingly potent immune therapies. A general way to reduce toxicity is to target immune potentiating ...

2017
Li Gao Qin Li Zebin Deng Brendan Brady Ni Xia Yang Zhou Haixia Shi

Molybdenum disulfide (MoS2) has shown highly attractive superiority as a platform for sensing. However, DNA physisorption on the surface of MoS2 was susceptible to nonspecific probe displacement and false-positive signals. To solve these problems, we have developed a novel MoS2-aptamer nanosheet biosensor for detecting thrombin using a covalently linked aptamer to the MoS2 nanosheet. Ten percen...

2014
Abdullah Ozer John M Pagano John T Lis

Single-stranded oligonucleotide aptamers have attracted great attention in the past decade because of their diagnostic and therapeutic potential. These versatile, high affinity and specificity reagents are selected by an iterative in vitro process called SELEX, Systematic Evolution of Ligands by Exponential Enrichment. Numerous SELEX methods have been developed for aptamer selections; some that...

2012
Wenhe Wu Jie Zhang Meiqin Zheng Yuhong Zhong Jie Yang Yuhong Zhao Wenping Wu Wei Ye Jie Wen Qi Wang Jianxin Lu

BACKGROUND An aptamer based biosensor (aptasensor) was developed and evaluated for rapid colorimetric detection of Escherichia coli (E. coli) O157:H7. METHODOLOGY/PRINCIPAL FINDINGS The aptasensor was assembled by modifying the truncated lipopolysaccharides (LPS)-binding aptamer on the surface of nanoscale polydiacetylene (PDA) vesicle using peptide bonding between the carboxyl group of the v...

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