Many B-cell lymphomas can be shown to contain cytoplasmic immunoglobulin which is characteristically monotypic with respect to light chains. In Hodgkin's disease, however, the Reed-Sternberg cells have been shown to contain both immunoglobulin light chains. This finding, which is also present in some other lymphomas, has been used as evidence both for and against a B-cell derivation of these ce...

Bone marrow involvement (BMI) is a well-known poor prognostic factor in patients with diffuse large B-cell lymphoma (DLBCL). This study robustly investigated the significance of monoclonal immunoglobulin gene rearrangement combined with histologic B-cell aggregates in bone marrow (BM) in the detection of a poor prognostic group. Pretreatment BM samples of 394 DLBCL patients were analyzed via th...

The dehydrogenation reaction of cholest-7-en-3P-ol (I) to cholesta-5,7-diem3/3-ol (II) in the presence of NADH was studied in rat liver microsomes and in microsomal acetone powder preparations, using [3Lu-“Hlcholest-‘7-en-3fi-o1. It was found that the reaction was inhibited by menadione, adenosine diphosphate, potassium ferricyanide, and cytochrome c while p-cresol had no effect. These results ...

Without intervention, a pregnant woman who is a chronic hepatitis B carrier is at risk of transmitting hepatitis B and of her infant becoming a chronic carrier and having a significantly increased lifetime risk of developing liver cancer or cirrhosis. Hepatitis B vaccine and immunoglobulin reduce the risk of the baby becoming a carrier, but with only a short window period after birth to deliver...

Nonviable cell preparations of Burkholderia mallei, the causative agent of glanders, were evaluated as potential vaccine candidates in a BALB/c murine model. Three different B. mallei cell preparations plus Alhydrogel were evaluated: a heat-killed preparation, an irradiation-inactivated preparation, and a preparation of a capsule-negative mutant strain which had been irradiation inactivated. BA...

Population studies at the b-locus of the "constant" regions of the rabbit immunoglobulin kappa 1 light chain (c kappa 1) revealed patterns of gene diversity resembling those that mark the peculiar nature of the major histocompatibility complex, such as large number of alleles, high heterozygosity levels, consistent excess of heterozygous individuals and long allele coalescence times. This paper...

A human plasma (inoculum one) containing hepatitis C virus (HCV) was passaged through eight chimpanzees in three generations. Of 10 HCV clones propagated from it, 7 were different in respect to the hypervariable region of E2/NS1 glycoprotein and they were named clones A, B, C, etc. A chimpanzee received inoculum one, and clone A accounted for 7 of the 10 clones from his acute-phase plasma (inoc...

OBJECTIVE Nyctereutes procyonoides immunoglobulin G (IgG) gene is partially cloned. MATERIAL AND METHODS In order to obtain a certain length (966bp) of Nyctereutes procyonoides immunoglobulin G (IgG), two pairs of primers are designed according to the conserved nucleotide sequence of canine (GenBank:AF354265, AF354265, AF354266, AF354267) and mink (GenBank: L07789). Using Bioinformatics techn...

A 76-year-old man presented with right shoulder pain, weight loss, and night sweats. Complete blood count showed isolated thrombocytopenia (26 3 10/L). The blood film demonstrated medium to large atypical lymphoid cells (12% of leukocytes), many with polylobated nuclei or flower cell morphology (panel A, image obtained using Olympus BX51 microscope, 31000 magnification). Computerized tomography...

The transpeptidase LtdMt2 catalyzes the formation of the (3-3) cross-links characteristic of the peptidoglycan layer in the Mycobacterium tuberculosis cell wall. Bioinformatics analysis suggests that the extramembrane part of the enzyme consists of three domains: two smaller domains (denoted as A and B domains) and a transpeptidase domain (the C domain) at the C-terminus. The crystal structures...