Callus induced from leaf explants of Pueraria lobata seedlings were suspended in Gamborg B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid, 1 mg l(-1) naphthalene acetic acid, 0.5 mg l(-1) kinetin and 30 g l(-1) sucrose. The effects of coconut milk and casein hydrolysate (CH) on cell growth and yields of puerarin and isoflavones in cells suspension were studied. The contents...

Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. T...

Water stress applied to the plant shoot through enhanced evaporative demands reduced cytokinin activity in extracts of xylem exudate and leaves. This reduction resembled the changes in cytokinin activity caused by water stress applied to the root. Cytokinin activity in detached wilting leaves decreased rapidly. Recovery took place after several hours in a humid chamber. Experiments with (14)C-k...

Teak planlets (Tectona grandis) of Salomon clones were irradiated by gamma rays to induce genetic variability for growth improvement. The objectives this research analyze the radiosensitivity T. grandis ray irradiation and variations using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD). For those purposes, teak at 5 different dosages, 0 Gy, 10 20 30 40 Gy. Lethal doses50 (LD50) reduc...

Pisang (Musa paradisiaca L.) adalah salah satu buah yang paling populer dan diminati oleh masyarakat Indonesia. Salah tanaman pisang memiliki potensi untuk dibudidayakan yaitu barangan. Upaya dalam menyediakan bibit unggul waktu singkat jumlah banyak satunya melalui kultur jaringan. ZPT (Zat Pengatur Tumbuh) Kinetin 2,4-D dikombinasikan dengan seimbang mampu memacu proses morfogenesis pada eksp...

chlorogenic acid, a pharmacologically important compound, is a phenolic compound that occurs in certain commonly used medicinal herbs. we looked for the presence of this compound in the callus cultures of varthemia persica dc (var. persica ). we have evaluated the conditions for establishment of callus cultures of v. persica and the in vitro production of chlorogenic acid. callus was initiated ...