We examined the role of endogenous tetrahydrobiopterin (BH4) on L-arginine (L-Arg)-induced increase in cytosolic calcium concentration ([Ca]i) in the β-cell line, HIT-T15 cells. HIT-T15 cells contain a large amount of BH4 with a high expression of GTP cyclohydrolase I (GTPCH) mRNA compared with mouse brain microvascular endothelial cells and bovine aortic endothelial cells. 2,4-Diamino-6-hydrox...

Cinobufagin (CBG), a major bioactive ingredient of the bufanolide steroid compounds of Chan Su, has been widely used to treat coronary heart disease. At present, the effect of CBG on the L-type Ca(2+) current (I Ca-L) of ventricular myocytes remains undefined. The aim of the present study was to characterize the effect of CBG on intracellular Ca(2+) ([Ca(2+)]i) handling and cell contractility i...

Lignite is an abundant carbon material with a variable surface structure and low cation density The introduction of metal (hydr)oxide phases has improved the anionic binding potential lignite. In this study, activated lignite (A−L), Ca2+−modified (Ca−L), (Ca−A−L) were synthesized to remove aqueous phosphate. was first (KOH: lignite, mass ratio, 3:1) at 750 ºC prepare A−L, improving its area by ...

This paper was aimed to study the effect of genistein (GST) on L-type calcium current (I(Ca,L)) in isolated guinea pig ventricular myocytes using whole cell patch-clamp recording technique. The results are as follows. (1) GST (10, 50, 100 micromol/L) reduced the voltage-activated peak amplitude of I(Ca,L) in a concentration-dependent manner. Daidzein (100 micromol/L), a structural analogue of G...

We reviewed the scientific literature addressing physiological constraints of zebra mussel and found seven studies that measured the effects of water calcium concentrations (mg/l, hereafter Ca) on zebra mussel development and growth (Jones and Ricciardi 2005, Allen and Ramcharan 2001, Hincks and Mackie 1997, Mellina and Rasmussen 1994, Ramcharan et al 1992, Strayer 1991, Sprung 1987). A 95% con...

Objective: Does carbachol (CCh) require NO/cGMP for inhibition of L-type calcium current (I ) when either adenylyl cyclase Ca(L) activation or phosphodiesterase suppression is used to raise cAMP? Methods: The effects of the NO donor SIN-1 (3-morpholinosydnonimine), CCh and atrial natriuretic peptide (ANP) were evaluated when I had been stimulated by isoproterenol (ISO) or Ca(L) 3-isobutyl-1-met...

L-type Ca(2+) channels play a critical role in regulating Ca(2+)-dependent signaling in cardiac myocytes, including excitation-contraction coupling; however, the subcellular localization of cardiac L-type Ca(2+) channels and their regulation are incompletely understood. Caveolae are specialized microdomains of the plasmalemma rich in signaling molecules and supported by the structural protein c...