BACKGROUND AND PURPOSE During the bladder filling phase, the volume of the urinary bladder increases dramatically, with only minimal increases in intravesical pressure. To accomplish this, the smooth muscle of the bladder wall must remain relaxed during bladder filling. However, the mechanisms responsible for the stabilization of bladder excitability during stretch are unclear. We hypothesized ...

It has been reported that the C0-C4 carbons of methionine are converted to ethylene in FMN-light (23) and Cu"!-ascorbate (10) model systems. Yang (22) has shown that C-C, carbons of 2-keto-4-methylthiobutyrate and the Cr, carbons of 3-methylthiopropionaldehyde (i.e., methional) are converted to ethylene in a model peroxidase system. When methionine was fed to apple tissue, the C-C4 carbons were...

One of the features of liver cirrhosis is an abnormal metabolism of methionine—a characteristic that was described more than a half a century ago. Thus, after an oral load of methionine, the rate of clearance of this amino acid from the blood is markedly impaired in cirrhotic patients compared with that in control subjects. Almost 15 y ago we observed that the failure to metabolize methionine i...

Unlike normal cells, malignant rat and two simian virus 40-transformed human cell lines can neither grow nor survive in B12- and folate-supplemented media in which methionine is replaced by homocysteine. Yet three lines of evidence indicate that the malignant and transformed cells synthesize large amounts of methionine endogenously through the reaction catalyzed by 5-methyltetrahydropterolyl-L-...

The production and spectroscopic properties of an L-selenomethionine-containing homolog of Pseudomonas aeruginosa azurin are described. The amino acid substitution was carried out by developing an L-methionine-dependent bacterial strain from a fully functional ATCC culture. Uptake studies monitored using L-[75Se]methionine indicated that L-selenomethionine was incorporated into the protein synt...

We examined effects of hyperhomocysteinemia on structure and mechanics of cerebral arterioles. We measured plasma total homocysteine (tHcy) and pressure, diameter, and cross-sectional area of the vessel wall in maximally dilated cerebral arterioles in heterozygous cystathionine -synthase–deficient (CBS / ) mice and wild-type (CBS / ) littermates that were provided with drinking water that was u...

Amino acids modulate mRNA translation through the 70 kDa ribosomal protein S6 kinase (S6K1) and the general control nondepressible 2 protein kinase (GCN2)/eukaryotic initiation factor 2 alpha eIF2 alpha pathways. The aim of the present study was therefore to explore the signaling cascades potentially modulated by methionine availability in quail muscle QM7 myoblasts using media providing all ot...

Materials. DL-Methionine-l-'4C (1.41 and 3.54 mCi/mmole), and DL-ornithine-l -†̃ †̃@ C (3.3 1 mCi/mmole) were obtained from New England Nuclear Corporation, Boston, Mass. The hydrochloride salts of putrescine, spermidine, and spermine and Tns, glutathione, and AlP were obtained from Sigma Chemical Company, St. Louis, Mo. S-Adenosyl-L-methionine and pyridoxal phosphate were obtained from Calbi...

this experiment was conducted in international researchery institute of sturgeon fishes shahid  dadman. the basal diet contained fish meal as protein source (cp=40%). five diets were supplemented with 5 graded levels of methionine, (0, 0.5,1 ,1.5, 2% met per kg diet) . each diet was randomly assigned to triplicate groups of 195 juvenile huso huso (initial weight 31.9± 2.8) in fiftin 500-l circu...

The mechanism of formation of the methyl group of methionine is of particular interest at the present time since the reaction may be studied in cell-free systems and is dependent on the direct participation of vitamin Bit. The reported systems for study of methionine biosynthesis in vitro have been derived from mammalian, avian, and bacterial sources (&lo). Serine and formaldehyde have been the...