In patients on long-term continuous ambulatory peritoneal dialysis, the efficiency of dialysis declines because of peritoneal neovascularization and loss of peritoneal mesothelial cells. In this study, we investigated the influence of lactate and bicarbonate in peritoneal dialysis fluid on such changes of the peritoneum. We studied the production of vascular endothelial growth factor (VEGF) and...

In glucose (Glc) metabolism, lactate dehydrogenase (LDH) functions by changing pyruvate into lactate. Lactate release is induced viacell death, suggesting to disease caused virus or pulmonary deterioration, such as inflammation of the lung triggered severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). A satisfactory evidence exists binding elevated levels development coronavirus 2019 (...

1. Two different gels have been prepared suitable for the separation of a number of enzymes, in particular NAD+-dependent dehydrogenases, by affinity chromatography. For both the matrix used was Sepharose 4B. For preparation (a), NAD+-Sepharose, 6-aminohexanoic acid has been coupled to the gel by the cyanogen bromide method and then NADI was attached by using dicyclohexylcarbodi-imide; for prep...

abstract objective removing plasma from the platelet concentrate (pc) medium could be an effective way to increase the safety of this product. the goal of this study was to compare pc stored in plasma or in an additive solution (composol) with in vitro testing. materials and methods fifty-four single donor pcs were prepared from iranian blood transfusion organization (ibto). each pc unit was di...

Studies on the mechanism of substrate interaction with rabbit muscle lactate dehydrogenase have recently been reported (1). These investigations, which were primarily kinetic in nature, indicated that nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide must add to the enzyme before the binding of substrates pyruvate and L-lactate. It was also noted that ternary compl...

Treatment with the polyene antibiotic, filipin, renders the spermatozoan cell membrane permeable to small molecules, but not to the intracellular enzymes aldolase and lactate dehydrogenase. Pyruvate (10 mM) as the sole substrate was metabolized very slowly. L-Carnitine increased pyruvate metabolism 3- to 4-fold and allowed limited rates of oxidative phosphorylation. When spermatozoa treated wit...

The accuracy of our lactate dehydrogenase (EC 1.1.1.27) isoenzyme procedure, based on electrophoresis in thin-layer agarose, has previously been assessed (Clin. Chem. 22: 1995, 1976). We now show that the method is similarly accurate and precise for the specific measurements of lactate dehydrogenase isoenzyme-1 and -2. We have also determined the lactate dehydrogenase isoenzyme pattern in perip...

introduction: busulfan as a chemotherapeutic agent causes testicular germinal epithelium depletion and cytotoxicity in germ cells. the aim of this study was to assess antioxidant status, reactive oxygen species (ros) generation and apoptosis-related genetic markers of adult male mouse sperm following busulfan treatment. materials and methods: forty adult nmri mice (30 ± 5 g) were divided into t...

Escherichia coli strains (KJ060 and KJ073) that were previously developed for succinate production have now been modified for malate production. Many unexpected changes were observed during this investigation. The initial strategy of deleting fumarase isoenzymes was ineffective, and succinate continued to accumulate. Surprisingly, a mutation in fumarate reductase alone was sufficient to redirec...