Microtubule assembly in vivo was studied by hapten-mediated immunocytochemistry. Tubulin was derivatized with dichlorotriazinylaminofluorescein (DTAF) and microinjected into living, interphase mammalian cells. Sites of incorporation were determined at the level of individual microtubules by double-label immunofluorescence. The haptenized tubulin was localized by an anti-fluorescein antibody and...

AIMS A statistical method used in ecology is adapted to characterize the degree of order in phyllotactic systems. SCOPE The test consists of subdividing a planar projection of the stem apical meristem into 16 sectors and counting the number of primordia appearing in each. By dividing the sum of squared deviations by the mean number of primordia per sector the chi-square (chi2) is obtained. Wh...

Expression of the K1 gene of human herpesvirus 8 activates nuclear factorB and induces lymph node hyperplasia and lymphomas in transgenic mice. To further delineate its role in cell survival, we determined whether K1 altered apoptosis of lymphoma cells. K1 protein is expressed in Kaposi sarcoma and primary effusion lymphoma. We retrovirally transfected BJAB lymphoma, THP-1, U937, and Kaposi sar...

PURPOSE To quantify the seasonal training load completed by professional soccer players of the English Premier League. METHODS Thirty players were sampled (using GPS, heart rate, and rating of perceived exertion [RPE]) during the daily training sessions of the 2011-12 preseason and in-season period. Preseason data were analyzed across 6×1-wk microcycles. In-season data were analyzed across 6×...

background patients, who survived from shotgun injuries, often have some retained lead pellets in their bodies. several cases of lead toxicity have been reported regarding these patients. objectives this study seeks to compare the serum lead level in patients who have retained lead pellets in their bodies with the control group. patients and methods in this case-control study, we gathered the s...

0