Low-density microarrays that utilize short oligos (<100 nt) for capture are highly attractive for use in diagnostic applications, yet these experiments require strict quality control and meticulous reproducibility. However, a survey of current literature indicates vast inconsistencies in the spotting and processing procedures. In this study, spotting and processing protocols were optimized for ...

Leaf, fruit, stem, bark and root of Zanthoxylum americanum were investigated for antifungal activity with 11 strains of fungi representing diverse opportunistic and systemic pathogens, including Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. All extracts demonstrated a broad spectrum of antifungal activity and inhibited at least eight fungal species in a disk diffusion ass...

Recent studies have detected phylogenetic signals in pathogen-host networks for both soil-borne and leaf-infecting fungi, suggesting that pathogenic fungi may track or coevolve with their preferred hosts. However, a phylogenetically concordant relationship between multiple hosts and multiple fungi in has rarely been investigated. Using next-generation high-throughput DNA sequencing techniques, ...

the effects of three arbuscular mycorrhizal fungi (amf) species (glomus versiforme, claroideoglomus etunicatum and rhizophagus intraradices) were studied on the growth parameters and nutrient uptake of three apple rootstocks (m.9, m.7 and mm.106). the soil medium contained a high level of calcium carbonate (38.65%). the results showed that amf inoculation could increase almost all growth parame...

tissue culture is one of the common methods for propagation of apple clones. however, low survival and poor growth of these plants, after transplanting, limit the widespread use of this technique. the success rate of this method can be increased by using bioagents such as arbuscular mycorrhizal fungi (amf). in this study, effect of mycorrhizal symbiosis and culture media on growth and establish...

Fungi regulate key nutrient cycling processes in many forest ecosystems, but their diversity and distribution within and across ecosystems are poorly understood. Here, we examine the spatial distribution of fungi across a boreal and tropical ecosystem, focusing on ectomycorrhizal fungi. We analyzed fungal community composition across litter (organic horizons) and underlying soil horizons (0-20 ...

The species diversity and distribution of 3 ecological groups of fungi (viz., foliar endophytes, phellophytes, and leaf litter fungi of 6 tree hosts of a dry thorn forest and 6 tree hosts of an evergreen forest of southern India) were studied. As the methods of sampling and the isolation procedure were maintained constant, the results could be compared across hosts and fungal assemblages. The d...

The anecic earthworm Lumbricus terrestris L. was kept in laboratory microcosms containing beech forest soil without litter, with beech leaf litter or with lime leaf litter. The structure of microfungal communities in soil, litter and fresh and aged (100 days) earthworm faeces was analysed using the washing and plating technique. The passage of mineral soil through the gut of L. terrestris affec...

Progress in understanding changes in soil biology in response to latitude, elevation and disturbance gradients has generally lagged behind studies of above-ground plants and animals owing to methodological constraints and high diversity and complexity of interactions in below-ground food webs. New methods have opened research opportunities in below-ground systems, leading to a rapid increase in...