An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with colloidal gold conjugated second antibody. The endogenous mammalian lectin was found in smooth muscle cells and squamous alveolar epithelial (type I) c...

Several Ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. A lectin from Penicillium chrysogenum, named PeCL, was purified from a fungal culture using gel-filtration chromatography column. PeCL was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visuali...

A novel lectin was isolated from leaves of the Japanese cycad, Cycas revoluta Thunb. (gymnosperm), and its characteristics including amino acid composition, molecular mass, carbohydrate binding specificity and partial amino acid sequences were examined. The inhibition analysis of hemagglutinating activity with various sugars showed that the lectin has a carbohydrate-binding specificity similar ...

A cDNA encoding a putative lectin expressed in tomato leaves was identified and analysed. The lectin consists of two homologous chitin-binding modules interconnected by a short proline-rich domain containing a single Ser[Pro]( n ) repetitive motif. Each module comprises two in-tandem-arrayed hevein domains separated by a tetrapeptide linker. Besides the chitin-binding modules and proline-rich d...

For the first time, the biological role of a lectin in the process of reaggregation of single cells from the same species (marine sponge: Geodia cydonium Jam.) is described. The galactose-specific lectin does not promote aggregation, but prevents the antiaggregation receptor from disaggregating cell clumps. Competition experiments showed that the lectin inactivates the antiaggregation receptor ...

1. Modification of potato (Solanum tuberosum) lectin with acetic anhydride blocked 5.1 amino and 2.7 tyrosyl groups per molecule of lectin and decreased the haemagglutinating activity of the lectin. De-O-acetylation regenerated 2.0 of the tyrosyl groups and resulted in a recovery of activity. 2. Modification with citraconic anhydride or cyclohexane-1,2-dione did not greatly affect activity, alt...

lectins first discovered more than 100 years ago in plants, they are now known to be present throughout nature. phytohemagglutinin (pha), the lectin extract from the red kidney bean ( phaseolus vulgaris ), contain potent, cell agglutinating and mitogenic activities. they play a role in biological recognition phenomena involving cells and proteins towards medical applications. the present articl...

Anthocyanins from the black soybean seed coat are known to have many pharmaceutical effects. However, contains antinutritional factors such as lipoxygenase, Kunitz trypsin inhibitor (KTI), lectin, and stachyose. The genetic removal of these components will improve nutritional value seed. objective this research was breed a strain with color, green cotyledon tetra recessive allele (lox1lox2lox3/...

Abstract Factor D (FD) is an essential element of the alternative pathway complement system, and it circulates predominantly in cleaved, activated form blood. In resting blood, mannose-binding lectin–associated serine protease 3 (MASP-3) exclusive activator pro-FD. Similarly to FD, MASP-3 also mainly active form. It was not clear, however, how zymogen activated. To decipher its activation mecha...

Cortical granule lectin (CGL) is released by the egg of the South African toad Xenopus laevis upon fertilization. The lectin binds to oligosaccharides in the extracellular matrix of the egg to form a physical block to prevent additional sperm penetration or polyspermy. To identify the oligosaccharides that bind to CGL, the lectin was immobilized on the surface of a matrix-assisted laser desorpt...