conclusions our data suggest that preconditioning with 5 μm h2o2 + 0.5% fbs and 10 μm h2o2 + 5% fbs improved cell proliferation and viability. however, the mechanisms related to protective properties of preconditioning and using this strategy in stem cell therapy requires more research. background mesenchymal stem cells (mscs) survival decreased following in vivo injection and its application h...

background: due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. in this study, we investigated the ability of human endometrial-derived stem cells (enscs) as a new source of mesenchymal stem cells to differentiate into adipocytes. enscs are the abundant and easy available source with no immunological response, for cell re...

Acute myeloid leukemia (AML) is characterized by an aberrant self-renewal of hematopoietic stem cells (HSC) and a block in differentiation. The major therapeutic challenge is the characterization of the leukemic stem cell as a target for the eradication of the disease. Until now the biology of AML-associated fusion proteins (AAFPs), such as the t(15;17)-PML/RARα, t(8;21)-RUNX1/RUNX1T1 and t(6;9...

BEGINNING AT THE BEDSIDE: A CASE OF T-CELL LEUKEMIA THIS STORY BEGINS with a seminal case report describing a 16-year-old boy who presented with acute lymphoblastic leukemia (ALL).1 He had an elevated white blood cell (WBC) count, thymic enlargement, and leukemic cells with an early T-cell phenotype (CD2 and CD7 positive; CD3, CD4 and CD8 negative). Standard chemotherapeutic agents failed to in...

Thymosin 8, (TB,) was originally isolated as a thymic hormone. Its functional properties remain obscure; however, the N-terminal peptidic sequence could have a regulatory function on hematopoietic stem cell proliferation. To investigate the mechanism of TB, expression, we studied TB, gene expression in various leukemic cells and in established cell lines. Among leukemic cell samples obtained fr...

The substance of bone marrow transplantation is the organ transplantation. Accurately, it is the grafting of hematopoietic and immunologic system. Comparing to the transplantation of solid organ, in the hematopoietic stem cell transplantation (HSCT), the ill organ, id est. hem‐ atopoietic and immunology system, is ablated by high-dose chemotherapy and total body irradiation(TBI) (conditioning r...

Bmi-1 and SALL4 are putative oncogenes that modulate stem cell pluripotency and play a role in leukemogenesis. Murine Sall4 also has been shown to play an essential role in maintaining the properties of ES cells and governing the fate of the primitive inner cell mass. Here, we demonstrate that transcription from the Bmi-1 promoter is strikingly activated by SALL4 in a dose-dependent manner by u...

Adult acute myeloid leukemia (AML) clinically is a disparate disease that requires intensive treatments ranging from chemotherapy alone to allogeneic hematopoietic cell transplantation (allo-HCT). Historically, cytogenetic analysis has been a useful prognostic tool to classify patients into favorable, intermediate, and unfavorable prognostic risk groups. However, the intermediate-risk group, co...