BACKGROUND AND PURPOSE Animal models with appropriate volume are crucial for preclinical assessment of aneurysm therapies. Our purpose was to control the aneurysm volume by adjusting the position of ligation during creation of elastase-induced aneurysms in rabbits. MATERIALS AND METHODS Sixty elastase-induced aneurysms in rabbits were created. Two different methods were used for creation, inc...

a series of complexes of the type trans-[co(acacen)(amine)2]clo4, where (acacen) is the (bis(acetyleacetone) ethylenediimine) and the amines are benzyl amine (bzlan) 1, 2-amino pyrimidine (2ampy) 2, n-methylpipirazine (nmpip) 3, 3-methyl pyridine (3mpy) 4, p-toluidine (p-toldn) 5, has been synthesized and characterized by uv-vis. and ft-ir. the kinetics of the substitution reaction of [co(acace...

Small, single-stranded DNA (ssDNA) circles have many applications, such as templating rolling circle amplification (RCA), capturing microRNAs, and scaffolding nanostructures. However, it is challenging to prepare ssDNA circles, particularly when the size becomes very small (e.g. a 20 nucleotide (nt) long circle). Often, short dominantly form concatemers (either linear or circular) due intermole...

Tandem ligation for the synthesis and modification of proteins entails forming two or more regiospecific amide bonds of multiple free peptide segments without a protecting-group scheme. We here describe a semi-orthogonal strategy for ligating three unprotected peptide segments, two of which contain N-terminal (NT) cysteine, to form in tandem two amide bonds, an Xaa-SPro (thiaproline), and then ...

XLF-Cernunnos (XLF) is a component of the DNA ligase IV-XRCC4 (LX) complex, which functions during DNA non-homologous end joining (NHEJ). Here, we use biochemical and cellular approaches to probe the impact of XLF on LX activities. We show that XLF stimulates adenylation of LX complexes de-adenylated by pyrophosphate or following LX decharging during ligation. XLF enhances LX ligation activity ...

Although reducing the temperature slows most chemical reactions, freezing can stimulate some reactions by mechanisms that are only partially understood. Here we show that freezing stimulates the self-ligation (circularization) of linear forms of the hairpin ribozyme (HPR) containing 2',3'-cyclic phosphate and 5'-OH termini. Divalent metal ions (M2+) are not required, but monovalent cations and ...

PURPOSE This study was designed to assess whether Doppler-guided hemorrhoid artery ligation can prevent patients from needing conventional surgery when rubber band ligation of their hemorrhoids has failed to achieve symptomatic relief. METHODS All patients who underwent treatment for hemorrhoids in two hospitals between September 2004 and June 2007 are reported. RESULTS A total of 203 patie...

INTRODUCTION Endoscopic band ligation (EBL) is superior to endoscopic injection sclerotherapy (EIS) of oesophageal varices, however, EBL is associated with a higher rate of variceal recurrences. OBJECTIVE To examine whether the reduction of recurrent varices can be achieved by additional sclerotherapy of remnant little varices after ligation. METHODS Forty-eight patients with liver cirrhosi...