A new system is presented for the generation of recombinant <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt...

Identification of the nucleotide sequence of the lipoprotein lipase gene as well as its role in the development of hyperlipidemia and pancreatitis in the Miniature Schnauzer Inaugural-Dissertation zur Erlangung der tiermedizinischen Doktorwürde

A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichi...

Lipase H (LIPH) is a membrane-bound phospholipase generating 2-acyl lysophosphatidic acid (LPA) in the body. LPA is a lipid mediator required for maintaining homeostasis of diverse biological functions and in activating cell surface receptors such as P2Y5, which plays an essential role in hair growth. Bioinformatic methods were used to predict the amino acid sequences, secondary and tertiary st...

the objective of the present study was to determine the antilipidperoxidation and in vitro α-amylase and lipase inhibitory activity of the boswellia ovalifoliolata bal henry (bob) extract. the polyphenolic compounds, flavonoids, and tannins content of the extracts were estimated by spectrophotometry. antioxidant activity on goat liver lipid peroxidation and linoleic acid was determined.moreover...

Since pancreatic cancer is an aggressive and often incurable malignancy, we investigated if the carboxyl ester lipase gene (CEL) is specifically expressed in pancreatic tissues and its promoter can be used for a specific suicide gene approach. Twenty-five tumor samples, 24 samples of normal pancreatic tissue and control tissues from other organs were examined by radioactive in situ hybridizatio...

Salicylic acid (SA) is a critical hormone for signaling innate immunity in plants. Here we present the purification and characterization of SA-binding protein 2 (SABP2), a tobacco protein that is present in low abundance and specifically binds SA with high affinity. Sequence analysis predicted that SABP2 is a lipase belonging to the alpha/beta fold hydrolase super family. Confirming this predic...

DNA fragments were amplified by PCR from all tested strains of Aeromonas hydrophila, A. caviae, and A. sobria with primers designed based on sequence alignment of all lipase, phospholipase C, and phospholipase A1 genes and the cytotonic enterotoxin gene, all of which have been reported to have the consensus region of the putative lipase substrate-binding domain. All strains showed lipase activi...

conclusions there was a significant correlation between elevated lipase levels and the incidence of multiple organ failure. moreover, no significant association was found between the elevated amylase levels and organ failure. however, on admission, measurement of these enzymes coupled with routine laboratory investigations can be a powerful tool in the early detection of patients progressing to...