The tissue-specific expression of lipoprotein lipase (LPL) in adipose tissue (AT), skeletal muscle (SM), and cardiac muscle (CM) is rate-limiting for the uptake of triglyceride (TG)-derived free fatty acids and decisive in the regulation of energy balance and lipoprotein metabolism. To investigate the tissue-specific metabolic effects of LPL, three independent transgenic mouse lines were establ...

The acute effects of intravenous heparin administration (50 U/kg body weight) on apolipoprotein (apo)B-48 and apoB-100-containing lipoproteins in relation to postheparin lipase activities were studied in ten healthy normolipidemic volunteers. Five subjects returned to receive sham injections with saline. Lipoproteins were separated from plasma by density gradient ultracentrifugation at baseline...

Lipoprotein lipase (LPL) is the enzyme responsible for the hydrolysis of plasma triglycerides from apolipoprotein C-II-containing lipoproteins at the capillary endothelium and it is synthesized in parenchymal cells of several tissues. Intracellular LPL processing is a major aspect of LPL regulation. The present study aims to determine the intracellular accumulation site of the LPL that is not g...

°C degrees Celsius 95% CI 95% confidence interval µg microgram ACTH adenocorticotropic hormone ALP alkaline phosphatase ALT alanine aminotransferase AST aspartate aminotransferase CETP cholesteryl ester transfer protein dL deciliter EDTA ethylenediaminetetraacetic acid GGT gamma-glutamyl transferase HDL high density lipoprotein IDL intermediate density lipoprotein L liter LCAT lecithin-choleste...

This study determines whether changes in abdominal (ABD) and gluteal (GLT) adipose tissue lipoprotein lipase (LPL) activity in response to a 6-mo weight loss intervention, comprised of a hypocaloric diet and low-intensity walking, affect changes in body composition, fat distribution, lipid metabolism, and the magnitude of weight regain in 36 obese postmenopausal women. Average adipose tissue LP...

Lipoprotein lipase (LPL) is widely linked to lipid and lipoprotein metabolism, but its effects on coronary artery disease (CAD) are not clearly elucidated. The aim of this study was to clarify the association between LPL gene polymorphisms and CAD susceptibility. The pooled odds ratio (OR) and 95% confidence interval (CI) were calculated to estimate the strength of the relationship between LPL ...

Rat hepatic triglyceride lipase (H-TGL) was purified from liver tissue extracts by affinity chromatography on Sepharose 4B with covalently linked heparin. The purified rat H-TGL exhibited the properties previously described for this enzyme. Enzyme protein was injected into rabbits for anti-H-TGL antibody production. Antirat-H-TGL did not react against rat lipoprotein lipase (LPL) but inhibited ...

This article is available online at http://www.jlr.org The lipolytic processing of triglyceride-rich lipoproteins (e.g., chylomicrons, very low density lipoproteins) by lipoprotein lipase (LPL) is the central event in plasma triglyceride metabolism and plays a crucial role in the delivery of lipid nutrients to parenchymal cells (e.g., adipocytes, myocytes) ( 1–4 ). LPL is synthesized by parench...

Lipoprotein lipase (LPL) hydrolyzes triglyceride in plasma lipoprotein primarily while bound to vascular endothelial cells. LPL metabolism by cultured endothelial cells was studied. Purified radioiodinated bovine LPL bound to porcine aortic endothelial cells at 4 degrees C with an association constant of 0.18 x 10(7) m-1. Analysis of the time course of LPL dissociation from endothelial cells at...

This article is available online at http://www.jlr.org The lipolytic processing of triglyceride-rich lipoproteins (e.g., chylomicrons, very low density lipoproteins) by lipoprotein lipase (LPL) is the central event in plasma triglyceride metabolism and plays a crucial role in the delivery of lipid nutrients to parenchymal cells (e.g., adipocytes, myocytes) ( 1–4 ). LPL is synthesized by parench...