We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

In the educational context it is examined the teacher-stress association. The teacher’s stress is related with variables of academic environment, of out of the school and of the teacher. The impact of that association can be see in the teacher’s health, in his achievement and in the quality of the teaching-learning process. With the objective to present one view of how issue is being considered...

We demonstrate that an oft-used indirect attitude assessment technique—the attitude activation paradigm—accurately assesses attitudes only when participants attend to the prime stimuli during the attitude activation task. Attitude activation attitudes toward obviously valenced words (e.g., torture, liberty) were more sensitive to attitude valence and extremity when participants were required to...

A new approach using microtiter plate cultivation with on-line measurement of dissolved oxygen (DO) was applied for medium optimization of mammalian cell culture. Applying dynamic liquid phase balance, oxygen uptake rates were calculated from the DO level and used as an indicator for culture viability. The developed method was successfully applied to optimization of the concentration of glucose...

The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample...

The aim of our research was to determine the influence of the soil type and adjuvants on the dynamics of ethofumesate degradation in soil. Samples of two types of soil were placed in growth chambers. Ethofumesate was applied at a rate of 800 g·ha, alone and in mixture with adjuvant based on methylated crop oil and surfactant adjuvant. Residues of ethofumesate were determined using high-performa...

High resolution magic angle spinning (HRMAS) has become an extremely versatile tool to study heterogeneous systems. HRMAS relies on magic angle spinning of the sample to average out to zero magnetic susceptibility differences in the sample and to obtain resonance linewidths approaching those of liquid state NMR. Shimming such samples therefore becomes an important issue. By analyzing the differ...

Fuel droplets in combustors interact strongly with flames and turbulence in practical liquid-fueled combustors of terrestrial and aerial propulsion systems (Williams 1965; Sirignano 1999). Although a great deal of physical understanding has been achieved in purely gaseous combustion over the last century, not much is currently known about spray combustion at the fundamental level. In this short...

INTRODUCTION The level of cotinine in biological specimens, such as serum, urine, and saliva, measured by gas or liquid chromatography is the most validated and reliable indicator of exposure to tobacco smoke. However, chromatographic methods are not always suitable for all types of situations. METHODS We validated a commercially available enzyme-linked immunosorbent assay (ELISA) that uses a...