In this study we propose a novel bacterial vaccine strategy where non-pathogenic bacteria are complemented with traits desirable for the induction of protective immunity. To illustrate the proof of principle of this novel vaccination strategy, we use the model organism of intracellular immunity Listeria. We introduced a, low copy number BAC-plasmid harbouring the virulence gene cluster (vgc) of...

The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121C for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintain...

The research was performed to investigate the effect of high hydrostatic pressure low temperature (HHPLT) on Listeria innocua in Ringer's solution (RS) and raw milk (RM). physicochemical properties RM were studied at 300 MPa 10 °C. Compared with control, protein lipase activities reduced HHPLT RM. The L-values (luminance) LHPLT applied RM significantly (P < 0.05) decreased for colou...

Listeria monocytogenes is a foodborne pathogen of significant concern to the agricultural and food processing industry because of its ability to grow and persist in cool and moist environments and its association with listeriosis, a disease with a very high mortality rate. Although there have been no listeriosis outbreaks attributed to fresh mushrooms in the United States, retail surveys and re...

Numerous cell surface components of Listeria influence and regulate innate immune recognition and virulence. Here, we demonstrate that lipidation of prelipoproteins in Listeria monocytogenes is required to promote NF-kappaB activation via TLR2. In HeLa cells transiently expressing TLR2, L. monocytogenes and Listeria innocua mutants lacking the prolipoprotein diacylglyceryl transferase (lgt) gen...

Colonization of barley plants by the food-borne pathogens Salmonella enterica serovar typhimurium and three Listeria spp. (L. monocytogenes, L. ivanovii, L. innocua) was investigated in a monoxenic system. Herbaspirillum sp. N3 was used as a positive control and Escherichia coli HB101 as a negative control for endophytic root colonization. Colonization of the plants was tested 1-4 weeks after i...

Two types of cold plasma generating paper-based electrodes (CPPE) made with flexible metallized paper were developed to decontaminate fresh produce. As opposed conventional rigid volume dielectric barrier discharge currently used for in-package generation which voltage increases as a function thickness, two CPPE configurations based on surface provided high local concentrations less was indepen...