AIM To compare the seminal attributes of neat, pre-freeze (at equilibration), and post-freeze (24 h after freezing) semen in pure and crossbred Jersey bulls. MATERIALS AND METHODS Total 36 ejaculates (3 ejaculates from each bull) were collected from 6 pure Jersey and 6 crossbred Jersey bulls and evaluated for various seminal attributes during neat, pre-freeze, and post-freeze semen. RESULTS...

The present study evaluated supplementation of zinc from different sources during chicken semen cryopreservation on post-thaw quality and fertility. Adult White Leghorn was cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). In the cryomixture oxide (6.25 12.5 µM) sulphate (100 200 added cryopreserved. plastic straws were thawed at 5°C for 100 sec samples sperm motility, li...

A new instrument for assessing mammalian semen attributes, the Sperm Quality Analyzer, was evaluated as a potential tool for determining rooster sperm quality. The Sperm Quality Analyzer measures the “activity” of sperm in a semen sample as the sperm motility index (SMI). The SMI is defined as the number and amplitude of deflections in a light path per second as a result of sperm movement wit...

Recently, numerous studies have reported that the mature sperm contains both coding and non-coding RNAs and the sperm delivers some RNAs to the oocyte at fertilization. However, the functions of the RNAs carried to the oocyte by sperm at fertilization in embryonic development remains a mystery. In this study, the mature spermatozoa were treated with lysolecithin, pronase and RNases (RNase A and...

Fifteen fertile rams (live weight 55.9 ± 1.21 kg, and 2.5-3 years old) were randomly allotted to three diets consisting of 0 (Control), 12.5 (PBP-12.5), and 25 % (PBP-25.5) pistachio by-products (PBP) in dry matter. Sperm motility parameters were detrimentally affected in animals feeding on PBP-25% diet (P<0.05). Sperm concentration of C17-1, C22-6-n-3, C18-2-Cis and C22-6 n-3 fatty acids decre...

Factors other than spermatozoa could be the major determinant of the success of assisted reproduction treatment in cases of male infertility. Our aim was to evaluate the effect of the wife's age and ovarian reserve on assisted reproduction success rates in the most severe type of male infertility, i.e. azoospermia. A total of 249 consecutive couples suffering from male infertility caused by azo...

Asia Pacific is experiencing multiple dynamics of urbanisation, economic growth, poverty and environmental deterioration. It is the largest region with more than 60 percent of the world population. About 43 percent of the total population in the region live in urban areas, of which 40 percent live in slums and substandard conditions. In the past 30 years, the urban population in Asia Pacific ha...

Micropuncture samples taken from the rete testis, caput, corpus and cauda epididymidis of adult rats were assayed for intraluminal sperm concentrations. The amount of fluid resorbed from the efferent duct and epididymal lumen was calculated based on the sperm concentration. Epididymal sperm concentrations increased from the rete fluid to the cauda fluid through the caput fluid. Eighty-nine perc...

Chicken seminal plasma contains a high content of sex steroids including progesterone (P4). According to a published study, in vitro supplementation of P4 to the semen resulted in decreased fertility in the White Leghorn chicken; however, it is not known if increased P4 production in vivo is associated with decreased sperm fertility. Therefore, the aim of the present experiment was to study the...

Motility parameters of the spermatozoa in most fish species spawning in fresh water like Rutilus kutum lasts for a short time after activation. Ovarian fluid significantly influenced sperm motility (motility duration period) and percent motility (progressive forward motile sperm). Both of these variables generally increased as the concentration of ovarian fluid increased from 33% to 50%, respec...