DNA sequencing by imaging in an electron microscope is an approach that holds promise to deliver long reads with low error rates and without the need for amplification. Earlier work using transmission electron microscopes, which use high electron energies on the order of 100 keV, has shown that low contrast and radiation damage necessitates the use of heavy atom labeling of individual nucleotid...

A study of inelastic interactions of low-energy electrons with biological media is of fundamental importance in the understanding of radiation-induced actions leading to the biological damage. In the present work, electron energy loss properties in liquid water and solid DNA were calculated based on the dielectric response theory for valence band electrons and atomic models for inner-shell elec...

Minnae Chabwera: NASA Undergraduate Research Scholar Advisor: Dr. Paul Guèye Institution: Hampton University Hampton, VA 23668 Introduction The research conducted under the guidance of Dr. Paul Guèye involved the construction of a Low Energy Electron Accelerator (LELIA) spearheaded by the Physics Department at Hampton University. The aim of this research has been to reactivate a thermionic gun...

A Geant4 hadronic process verification suite has been designed to test and optimize Geant4 hadronic models in the cascade energy range. It focuses on quantities relevant to the LHC radiation environment and spallation source targets. The general structure of the suite is presented, including the user interface, stages of verification, management of experimental data, event generation, and compa...

In low-pressure capacitively coupled plasmas, high-energy electrons are collisionlessly heated by large rf fields in the sheaths while low-energy electrons are confined in the bulk plasma by the ambipolar potential. Low-energy electrons are typically inefficiently heated due to their low collisionality and the weak rf electric field present in the bulk. It is shown, however, that as a result of...

Radiation damage is considered to be the major problem that still prevents imaging an individual biological molecule for structural analysis. So far, all known mapping techniques using sufficient short wavelength radiation, be it x rays or high energy electrons, circumvent this problem by averaging over many molecules. Averaging, however, leaves conformational details uncovered. Even the antici...