There is a discontinuum of hereditary and acquired disorders of phosphate homeostasis that are caused by either high or low circulating levels of the novel phosphaturic hormone fibroblastic growth factor 23 (FGF23). Disorders that are caused by high circulating levels of FGF23 are characterized by hypophosphatemia, decreased production of 1,25-dihydroxyvitamin D, and rickets/osteomalacia. On th...

In this paper, a method of Micellar Enhanced Ultrafiltration (MEUF) with Hexadecyltrimethylammonium bromide (CTAB) is proposed to remove phosphate ions from treated domestic wastewaters from the reclamation station of University Campus of Toledo (Spain). This technique is able to reach phosphate rejection coefficients higher than 99% (a phosphate concentration in permeate of 1 mg/L if feed conc...

Phosphate, 3 - 10 nmol, in 1 dm3 of natural-water samples was quantitatively collected along with 10 micromol of Fe(III) ion onto a silica-gel collector in the pH range of 5.4 - 6.2. The amount of Fe(III) ion needed was limited to such a low level that the Pyrocatechol Violet method could be applied without removing the Fe(III) ion, providing a superior determination method for phosphate. Surfa...

Several bacterial response regulator proteins (CheY, NRI, PhoB, and OmpR) become phosphorylated in vitro when incubated with acetyl phosphate. In the presence of high levels of acetyl phosphate and Mg2+, CheY reached steady state phosphorylation in less than 30 s; NRI and PhoB reached steady state more slowly (t1/2 to steady state of 1.5 and > 15 min, respectively). A simple method was develope...

Cells must rapidly adapt to changes in nutrient availability. In budding yeast, limitation of phosphate rapidly induces the expression of the Pho regulon genes [1-4]. This starvation program depends on the transcription factor Pho4, which translocates to the nucleus within minutes when cells are transferred to a low-phosphate medium [5]. Contrasting its rapid induction, we report that the Pho r...

Glucose-6-phosphate dehydrogenase (G6PD) in humans is an X-chromosome-linked disorder and housekeeping enzyme, vital for the survival of every cell. It catalyses the oxidation of glucose-6-phosphate to 6-phospho gluconate in the first committed step of the pentose phosphate pathway, which provides cells with pentoses and reducing power in the form of NADPH. NADPH is required to protect the cell...

MATERIALS AND METHODS Estramustine phosphate (0.01 to 0.5 HIM),an estradici mustard derivative used in the therapy of prostatic carcinoma, inhibited the assembly of brain microtubule proteins in vitro and disassem bled preformed microtubules. In the presence of estramustine phosphate, the minimum microtubule-protein concentration suf ficient for the assembly of microtubules was increased. Low c...

Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-...

We use dibutyl phosphate to simulate the behavior of the phosphate group in DNA towards the attack of low energy electrons. We find that the compound undergoes effective dissociative electron attachment within a low energy resonant feature at 1 eV and a further resonance peaking at 8 eV. The dissociative electron attachment (DEA) reactions are associated with the direct cleavage of the C-O and ...