نتایج جستجو برای: lung isolation
تعداد نتایج: 437467 فیلتر نتایج به سال:
Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133+ phenotype was successfully isolated from a single cell suspension of lung adenocarcinoma tissue using magnetic-...
background: foundry workers are occupationally exposed to hazardous substances such as silica dusts and toxic gases. the aim of this study was to examine the effects of simultaneous exposure to complex mixtures of silica dust, formaldehyde, and triethylamine on lung function parameters. study design: a cross-sectional study. methods: this study was conducted on 55 male workers of core making un...
obesity as a common health risk is increasing all over the world. the aim of this study was evaluation of standing and sitting positions on spirometric values in obese asthmatic patients, in comparison with normal obese subjects. the study included 49 obese asthmatic patients with mean age of 42.63 years and body mass index of 36.06 kg/m 2 , and 51 control obese normal subjects with mean age of...
Clara cells are involved in the cytochrome P-450-dependent metabolism of certain lung toxins, e.g. ipomeanol [I], but studies have mainly been on their action on whole lung tissue. Attempts to isolate Clara and other lung cells have in the past produced only partial enrichment owing to the limitations of purification by density gradients and centrifugal elutriation [2, 31, although, more recent...
A method is described for isolating Clara cells from the mouse lung that does not require the technique of elutriation. Mouse lungs totally perfused of blood are instilled with crystalline trypsin (0.25%) and incubated for the optimum time of 15 min. The lung tissue is chopped, mechanically agitated, and sequentially filtered to obtain a primary digest of 3 to 5 x 10(6) cells. Clara cells, iden...
In breast tumorigenesis, the metastatic stage of the disease poses the greatest threat to the affected individual. Normal breast cells with altered genotypes now possess the ability to invade and survive in other tissues. In this protocol, mouse mammary tumors are removed and primary cells are prepared from tumors. The cells isolated from this procedure are then available for gene profiling exp...
Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studie...
Lung cancer is a malignant lung tumor with various histological variants that arise from different cell types, such as bronchial epithelium, bronchioles, alveoli, or bronchial mucous glands. The clinical course and treatment efficacy of lung cancer depends on the histological variant of the tumor. Therefore, accurate identification of the histological type of cancer and respective protein bioma...
in spite the emergence of potent and broad spectrum antibiotics and recent advances in bronchoscopy and pulmonary physiotherapy, still there is a high rate of morbidity and mortality because of lung abscess. the objective of this study is the indication of risk factors, which have undesirable effects on the prognosis of lung abscess. in a retrospective study, all cases of lung abscess who was c...
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