Escherichia coli phenylalanyl-tRNA synthetase, a-subunit (ePheS) can be useful as a counterselection marker since its A294G variant misincorporates 4-chloro-phenylalanine (4CP) into cellular proteins during translation, thereby causing cell death. The drawback of this method is that selection must be performed in minimal or semisynthetic medium to avoid interference from phenylalanine in the me...

Estimation methods for mutation rates (or probabilities) in Luria-Delbrück fluctuation analysis usually assume that the final number of cells remains constant from one culture to another. We show that this leads to systematically underestimate the mutation rate. Two levels of information on final numbers are considered: either the coefficient of variation has been independently estimated, or th...

Statphylococcus aureus is a Gram positive bacterium, which is naturally present in nasopharyngeal region of human beings and animals. After 1941, S.aureus was developed drug resistant capacity to against all penicillin antibiotics. In 1961, Methicillin Resistant Staphylococcus aureus were isolated. The present study Methicillin Resistant S. aureus were isolated from clinical pus samples. All is...

Cognitive deficit is present in most of schizophrenia cases and even better explains functional outcomes then positive and negative symptoms. There have been less consensus regarding the long-term course of cognitive functioning after onset of the illness. In our study we used a neuropsychological test battery based on Luria`s systematic approach in testing of patients at their first episode of...

Pseudomonas fluorescens GcM5-1A, isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, was cultured in Luria Broth medium (LB). The clarified culture was extracted with ethyl acetate, and two dipeptides were purified from the extract. The chemical structures of 1 and 2 were identified as cyclo(-Pro-Val-)and cyclo(-Pro-Tyr-), respectively, by MS, (1)H NMR, (13)C NMR,(1)H-(1)H C...

The author presents here the results of a series of objective experiments conducted by him at the State Institute of Experimental Psychology, Moscow, during the years 1923-30, and devised to investigate certain aspects of the disorganization of behavior during emotional stress. Luria rejects the postulates of Pavlov and other conditioned reflex adherents and aligns himself with that group repre...

Serratia marcescens has been studied to show a higher degradation potential for organo-phosphorus pesticides. This property made it useful as bioremediation agent. The bacterial cell was bio-stimulated in luria beterni broth at selected experimental temperature ranging from 25 ºC – 40 ºC. highest biomass recorded 35 range 7.56 -8.10mg/ml single strength and double yeast extract beterni, respect...

Originating from the Luria–Delbrück experiment in 1943, fluctuation analysis (FA) has been well developed to demonstrate random mutagenesis microbial cell populations and infer mutation rates. Despite remarkable progress its theory applications, FA often faces difficulties computation perspective, due lack of appropriate simulators. Existing simulation algorithms are usually designed specifical...