نتایج جستجو برای: lyophilization technique
تعداد نتایج: 612174 فیلتر نتایج به سال:
Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs) and lipid-core nanocapsu...
The present study focused on the development of three layered small-diameter (<6 mm) extracellular matrix (ECM)-based vessels. These were engineered artificially through the freeze-drying technique. A layer of decellularized bovine aorta (DAM) was deposited on a mandrel and, after lyophilization, it was dipped into a poly-L-lactide acid (PLLA)/polyethylene glycol (PEG) 2000 dichloromethane solu...
Bone changes its structural and physical properties during maturation. In order to validate ultrasound measurements with regard to their usefulness in assessing cortical bone maturation, speed of sound (SOS) data were compared with mechanical properties (elastic modulus, bending strength, and cortical surface hardness), density and water content. Thirty pig mandibles were selected from three di...
The lethal effect of oxygen on Escherichia coli organisms freeze-dried from water may be partly abolished by the addition of certain compounds before lyophilization. Three groups of such protective compounds were found: (i) thiourea, some of its derivatives and analogues; (ii) sugars, especially monosaccharides and some of their derivatives; (iii) some simple inorganic salts. The protective act...
The growth curves of strains belonging to genera Candida and Saccharomyces grown through surfaced and submerged cultivation and their survival after lyophilization have been studied. Conclusions of practical importance have been drawn. It was found out that three-day-old cultures cultivated in solid medium possessed maximal resistance to freeze-drying. Nevertheless the cultures obtained by subm...
Purpose: Psylliumseeds are used in traditional herbal medicine to treat various disorders. Moreover, as a soluble fiber, psyllium has potential to stimulate bacterial growth in digestive system. We aimed to substitute alkali-extractable polysaccharides of psyllium for alginate in beads with second coat of poly-l-lysine to coat Lactobacillus acidophilus. Methods: Beads were prepared using extrus...
We describe a radiochemical method for measuring ferrochelatase activity in the in vitro incorporation of iron into mesoporphyrin-IX to form mesoheme. 59Fe is used to quantify ferrochelatase activity in rat liver. The uptake of iron is linearly related to the time allowed for it to occur, and the reaction proceeds optimally under reducing and anerobic conditions, maintained in sealed lyophiliza...
conclusions employing the lpe as a new approach, gives novel concept of probiotic-products in food and medical marketing. results results showed that, lpe had more antipathogenic effects, antioxidant activity, and stability during storage-time when compared to fresh probiotic-extract. objectives in this study extract of cultured probiotics without cells was evaluated for its antimicrobial effec...
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